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一株抑制浒苔生长海洋细菌的分离与鉴定
汪靖超1, 辛宜轩2
1.青岛大学生物系;2.青岛第二中学
摘要:
从青岛近海海泥中分离了57 株细菌, 通过毒性实验, 得到了一株对浒苔具有较强毒性的细菌EP23。对该菌株进行了菌落、菌体形态学观察、生理生化特征分析及16S rDNA 序列分析, 鉴定该菌为弯曲芽孢杆菌(Bacillus flexus)。该菌培养液高速离心的上清液对浒苔具有较强毒性, 表明弯曲芽孢杆菌EP23 是通过分泌胞外物质的间接方式进行的。
关键词:  溶藻细菌  浒苔(Enteromopha prolifera (Müller) J. Ag.)  鉴定  弯曲芽孢杆菌(Bacillus flexus)
DOI:
分类号:
基金项目:
Isolation and identification of a bacterial strain that inhabits the growth of Enteromopha prolifera
Abstract:
Fifty-seven bacteria strains were isolated from sea mud at Qingdao. Strain EP23 was found to have algae-lytic activity toward Enteromopha prolifera. Strain EP23 was identified as Bacillus flexus by morphological characters, physiological characteristics, and 16S rDNA sequence analysis. After ultracentrifugation, the bacterial culture supernatant displayed algae-lytic activity toward Enteromopha prolifera, indicating that certain extracellular substances produced by the strain are responsible for the algae-lytic ability.
Key words:  algae-lysing bacterium  Enteromopha prolifera  identification  Bacillus flexus
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