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海洋交替单胞菌YTW-10的鉴定及抑菌活性分析
王 磊1, 宿红艳1, 杨润亚1, 王晓杰1, 林文杰1
鲁东大学生命科学学院
摘要:
菌株YTW-10分离自山东烟台海域近海盐场泥样中。该菌能在NaCl浓度为1%～15%的培养基中生长, 最适生长NaCl浓度为7%,最适生长温度为37 ℃, 最适pH值为8。经过形态学观察、生理生化特性分析及基于16S rDNA的系统进化分析, 该菌株鉴定为交替单胞菌属。以3株水产生物致病细菌和3株农作物致病真菌作为指示菌, 检测其抑菌活性。实验结果表明, YTW-10对小麦赤霉病菌、西瓜枯萎病菌和玉米弯胞病菌有明显的抑制效果。
关键词:  交替单胞菌  抑菌活性  鉴定
DOI：
分类号:
基金项目:山东省高等学校科技计划项目(J09LC01); 山东省优秀中青年科学家科研奖励基金(BS2010SW035)
Identification and antimicrobial activity analysis of a marine Alteromonas YTW-10
Abstract:
The strain YTW-10 was isolated from marine sediments collected from the Yantai beach. It was able to grow in the medium containing 1%～15% NaCl with the optimal growth salinity of 7%. The optimum growth temperature was 37 ℃ and the initial pH value was 8. Based on morphology, biochemical characteristics, as well as phylogenetic analysis, the strain YTW-10 was identified as Alteromonas. Three marine pathogenic bacteria and three crop pathogenic fungi were used as indicators in YTW-10 antimicrobial experiment. The result showed that YTW-10 had strong antimicrobial activity to Curvlaria lunata, Fusarium ozysporum, and Fusarium graminearum Schw.
Key words:  Alteromonas  antimicrobial activity  identification
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