| 摘要: |
| 用乙炔抑制法和最大或然数(most probable number, MPN)法对黄海北部海域沉积物反硝化速率及反硝化细菌数量的季节变化进行了研究, 结果表明, 该海域反硝化速率在夏季最大, 范围在3.2～7.5μmol/(m2·h)之间, 平均值为4.85μmol/(m2·h); 而在春、秋季其范围分别为0.26～2.65μmol/(m2·h)和1.21～4.12μmol/(m2·h)。该研究海域3 个季节反硝化细菌数量差别较大, 春、夏、秋季分别在1.78×104～8.12×104, 1.18×106～6.18×106 和0.72×105～4.50×105 个/g 之间。春、秋两季反硝化速率和反硝化细菌数量之间呈显著性正相关, 相关系数分别为0.759 和0.750(P<0.05)。本结果可为黄海北部海域氮循环机制研究提供重要参考。 |
| 关键词: 反硝化细菌 反硝化速率 季节变化 北黄海 |
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| 基金项目:国家水体污染控制与治理科技重大专项(2008ZX07208-009); 国家重点基础研究发展计划(973 计划项目2006CB400602) |
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| Seasonal variation of denitrifying bacteria and denitrification rate in the sediment of the northern Yellow Sea |
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| Abstract: |
| The denitrification rate and denitrifying bacteria in sediment were measured using acetylene inhibition technique and most probable number (MPN) method in different seasons in the northern Yellow Sea. The denitrification rates ranged from 3.2 to 7.5 μmol/(m2·h) with the average of 4.85 μmol/(m2·h). The rates were 0.26 to 2.65 μmol/(m2·h) and 1.21 to 4.12 μmol/(m2·h) in spring and autumn, respectively. The denitrifying bacteria were 1.78×104～8.12×104, 1.18×106～6.18×106 and 0.72×105～4.50×105 cells/g in spring, summer and autumn, respectively. The result also showed that there were significant positive relationship between denitrification rate and denitrifying bacteria, particularly in spring and autumn. The correlation coefficients were 0.759 and 0.750 (P< 0.05) in spring and autumn. The results provided evidences for elucidation of the nitrogen cycling mechanism in the northern Yellow Sea. |
| Key words: denitrifying bacteria denitrification rate seasonal variation the northern Yellow Sea |
