| 摘要: |
| 利用彗星电泳技术, 研究了不同胁迫浓度和胁迫时间下, Cd2+和Hg2+对四角蛤蜊(Mactra veneriformis)血细胞DNA 损伤的情况。研究结果显示, 50、100 和200 μg/L Cd2+胁迫14 天不能造成四角蛤蜊血细胞DNA 损伤。10、20 和40 μg/L Hg2+胁迫均能明显损伤血细胞DNA, 并且DNA 损伤程度分别与Hg2+胁迫时间、胁迫浓度具有一定正相关性。200 μg/L Cd2++20 μg/L Hg2+复合胁迫组, 四角蛤蜊血细胞DNA 损伤程度随胁迫时间延长而增加, 并且大于Hg2+胁迫组。上述结果表明对于四角蛤蜊而言, Hg2+潜在的遗传毒性大于Cd2+; Cd2+和Hg2+对DNA 的损伤具有协同作用。 |
| 关键词: 四角蛤蜊(Mactra veneriformis) Cd2+ Hg2+ DNA 损伤 彗星电泳 |
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| 基金项目:国家基金委创新研究群体科学基金项目(40821004); 国家973计划资助项目(2007CB407305); 国家农业部公益性计划资助项目(nyhyzx07-047); 国家海洋公益性行业科研专项资助项目(200805069) |
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| Effects of Cd2+ and Hg2+ on DNA damage in haemocytes of Mactra veneriformis |
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| Abstract: |
| The objective of this study was to investigate the effects of Cd2+ and Hg2+ on DNA damage in haemocytes of Mactra veneriformis that were exposed to different concentrations of Cd2+ or/and Hg2+ for 14 days. DNA damage was determined by the comet assay. There was no obvious DNA damage in haemocytes caused by 50, 100, and 200 μg/L Cd2+ during the exposure period. However, Hg2+ caused significant DNA damage in haemocytes at doses of 10, 20, and 40 μg/L in a dose- and time- dependent manner. Combination of 200μg/L Cd2+ and 20μg/L Hg2+ induced a time-dependent increase of DNA damage. The degree of DNA damage in the combined exposure group was severer than that in Hg2+-treated groups. These results indicate that the ranking of genotoxic potential to M.veneriformis is in decreasing order: Hg2+>Cd2+. There is a synergistic effect to DNA damage between Cd2+ and Hg2+. |
| Key words: Mactra veneriformis Cd2+ Hg2+ DNA damage comet assay |
