| 摘要: |
| 以新鲜缢蛏(Sinonovacula constricta)为材料, 经水萃取、(NH4)2SO4盐析、DEAE 离子交换层析、Sepharose CL-6B 凝胶层析、冻干浓缩。结合SDS-PAGE 电泳技术, 硫代巴比妥酸(TBA)法初步鉴定各个组分的抗氧化活性, 并用Bradford 法进行蛋白的定量。结果表明, 80%饱和度(NH4)2SO4 盐析得到的组分经离子交换层析以及凝胶层析得到一个蛋白纯品YC-2, 具有2 个亚基, 大亚基的分子质量为61.23ku, 小亚基的分子质量为45.39 ku, YC-2 蛋白的分子质量为106.62 ku, 而且其具有较高的抗氧化活性,在0.627 g/L 时对·OH 清除率达99.7 %。 |
| 关键词: 缢蛏(Sinonovacula constricta) 蛋白质 分离纯化 抗氧化活性 |
| DOI： |
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| 基金项目:江苏省海洋生物技术重点建设实验室开放基金项目(HS07014); 江苏省基础研究计划(自然科学基金)——企业博士创新项目(BK2009641); 淮海工学院引进人才科研启动基金( KQ07018) |
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| Extraction of YC-2 from Sinonovacula constricta and its antioxidant activity |
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ZHAO Yan-jing,PEI Bo,HU Hong,WANG Ying
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| Abstract: |
| Protein YC-2 was isolated from Sinonovacula constricta by water extraction, ammonia sulphate precipitation (80% saturation), ion exchange chromatography, and gel chromatography, and concentrated by freeze drying. SDS-PAGE and the thiobarbituric acid (TBA) method were used to identify various components and their antioxidant activities in Sinonovacula constricta extracxts. We found that YC-2, a dimeric proteins with a 61 ku large subunit and a 45 ku small subunit, had a high oxidation resistance, the removal rate on ? OH being 99.7 % at 0.627 g/L. |
| Key words: Sinonovacula constricta protein separation and purification antioxidant activity |
