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马粪海胆生殖腺粗多糖提取工艺研究
牛宗亮1, 王荣镇1, 董新伟1, 郭承华1
烟台大学 化学生物理工学院
摘要:
采用L9(34)正交实验, 以马粪海胆(Hemicentrotus pulcherrimus)生殖腺粗多糖得率为指标, 研究提取温度、提取时间、料水比、提取次数4 个因素对马粪海胆生殖腺粗多糖提取工艺的影响。以正交实验的实验条件和马粪海胆生殖腺粗多糖得率为自变量和因变量建立多元线性模型, 所得到的统计参数分别为R=0.922, F=2.854, 并用所建立的线性模型预测两个实验条件的多糖得率。
关键词:  马粪海胆(Hemicentrotus pulcherrimus)  粗多糖  正交实验  提取工艺  多元线性回归
DOI:
分类号:
基金项目:山东省自然科学基金资助项目(Y2008E05)
Optimizition of extracting crude polysaccharides from the gonad of Hemicentrotus pulcherrimus
NIU Zong-liang,WANG Rong-zhen,DONG Xin-wei,GUO Cheng-hua
Abstract:
The extraction temperature, extraction time, ratios of starting material/water and times of extraction were selected as variables in the L9(34)experimental design. Their different effects on the yield of polysaccharides extracted from the gonad of Hemicentrotus pulcherrimus were studied. In addition, a multiple linear regression model, in which the yield of polysaccharide was a function of the experiment conditions of the orthogonal experiments, was constructed to simulate the yields of polysaccharides under two experimental conditions. The statistic parameters were R = 0.922 and F= 2.854.
Key words:  Hemicentrotus pulcherrimus  crude polysaccharide  orthogonal experiment  extraction process  multiple linear regression
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