| 摘要: |
| 以热酚水抽提法提取致病溶藻弧菌(Vibrio alginolyticus)粗脂多糖(Lipopolysaccharides, LPS), 并将不同浓度的溶藻弧菌粗LPS 通过腹腔注射法接种点带石斑鱼(Epinephelus coioides), 研究该LPS 对点带石斑鱼毒性和免疫原性的影响, 并同弱致病溶藻弧菌粗LPS 及高纯度大肠杆菌(Escherichia coli)LPS对石斑鱼的刺激效果进行比较。结果表明: 溶藻弧菌致病株和弱致病株粗LPS 均对石斑鱼具有比较强的毒性, 溶藻弧菌LPS 对石斑鱼的免疫原性随LPS 浓度的增高而增强, 高纯度大肠杆菌LPS 对石斑鱼的免疫原性效果要优于溶藻弧菌粗LPS。
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| 关键词: 溶藻弧菌(Vibrio alginolyticus) 脂多糖 毒性 免疫原性 |
| DOI： |
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| 基金项目:国家自然科学基金项目(30660144); 新世纪优秀人才支持计划项目(NCET-05-0755); 海南大学2009 年度科研项目(hd09xm57) |
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| Toxicity and immunogenicity of LPS from pathogenic Vibrio alginolyticus to grouper, Epinephelus malabaricus |
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XU Xian-dong,XIE Zhen-yu,WANG Shi-feng,XUAN Xiong-zhi,ZHOU Yong-can
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| Abstract: |
| In order to study the toxicity and immunogenicity of the LPS from pathogenic Vibrio alginolyticus to Epinephelus malabaricus, the crude LPS was extracted by hot phenol-water extraction, and was intraperitoneally injected to healthy groupers at different concentrations. It was found that crude LPSs from both the pathogenic strain and the weak pathogenic strain of V. alginolyticus were highly toxic to groupers. The immunogenicity of the crude LPS from V. alginolyticus to the groupers was enhanced at elevated concentrations of LPS. The immunogenicity of high purity LPS from E.coli to the groupers was better than the crude LPS from V. alginolyticus.
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| Key words: Vibrio alginolyticus lipopolysaccharides toxicity immunogenicity |
