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刺参肌肉组织cDNA文库的构建
陈璐1, 姜国良1, 刘云1, 仇磊1, 项鹏1
中国海洋大学
摘要:
用RNA提取试剂——TRIZOL Reagent提取刺参(Apostichopus japonicus)肌肉组织总RNA，用SMART cDNA Library Construction Kit构建cDNA文库。经测定原始文库滴度达到 3.2×106，扩增后文库滴度达到 5.1×109，重组率达到96.7%，从扩增文库随机挑取12 个克隆进行PCR 扩增鉴定,结果显示,插入片段大小为0.5～2.5 kb。通过各项指标验证成功构建了刺参肌肉组织cDNA 文库。
关键词:  刺参(Apostichopus japonicus)  肌肉组织  cDNA文库
DOI：
分类号:
基金项目:国家自然科学基金项目(30571417)
Construction of cDNA library from the musculature of Apostichopus japonicus
CHEN Lu,JIANG Guo-liang,LIU Yun,QIU Lei,XIANG Peng
Abstract:
Total RNA of muscles of Apostichopus japonicus were extracted by TRIZOL Reagent. cDNA library was constructed with SMART cDNA library construction kit. The primary library hada high titer of 3.2×106, in which 96.7% clones were recombinant. The amplified library hada titer of 5.1×109, and the length of most cDNA inserted in the library was about 0.5～2.5 kb. It was concluded that the cDNA library of Musculature of A. japonicus had been constructed by using the SMART technique.
Key words:  Apostichopus japonicus  muscle  cDNA library
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