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有棘无棘两种表型栉江珧28S和COI基因序列差异的比较
薛东秀1,2, 张 涛2, 王海艳2, 郑小东1, 孟凡玉3
1.中国海洋大学;2.中国科学院海洋研究所;3.江苏省赣榆县柘汪镇渔业指导站
摘要:
在形态学分析的基础上，对有棘和无棘两种不同表型栉江珧(Atrina pectinata)的28S rDNA 和COI基因片段进行序列分析比较。结果显示这两种表型的DNA序列差异很小，28S rDNA（1 075 bp）无差异，而COI（659 bp）碱基差异最大为1.5%，不能提供这两种表型的栉江珧划分为两个种的证据。
关键词:  栉江珧(Atrina pectinta)  棘  28S rDNA  COI
DOI：
分类号:
基金项目:江苏省科技支撑计划项目(农业部分，合同号：BE2008344)；国家海洋局公益性行业科研专项重点项目(200805069)
Comparative study on 28S rDNA and COI of scaly and non-scaly phenotypes in species of Atrina pectinata
XUE Dong-xiu,ZHANG Tao,WANG Hai-yan,ZHENG Xiao-dong,MENG Fan-yu
Abstract:
The ANOVA analysis was conducted to the length, height and width of shells of scaly and non-scaly phenotypes in species of Atrina pectinata. We use DNA sequence data from the nuclear 28S rDNA and mitochondrial cytochrome oxidase I (COI). The length of sequenced 28S rDNA genes is 1 075 bp, and COI's is 659 bp. There are little differences between the DNA sequences of the two types of A. pectinata. It is suggested that the two types of A. pectinata should belong to the same species.
Key words:  Atrina pectinata  Scaly  28S rDNA  COI
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