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| 不同碳源富集的石油烃降解菌群结构的分析 |
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杨仕美1,2, 张翼霄1,2, 高光军3, 郭利果1,2, 梁生康1,2, 苏荣国1,2
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1.中国海洋大学;2.海洋化学理论与工程技术教育部重点实验室;3.中国石油化工股份有限公司胜利油田分公司
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| 摘要: |
| 以原油和正十六烷为唯一碳源，从长期受石油污染的环境中富集和分离具有不同功能的石油烃降解菌，并利用平板法和PCR-DGGE法对不同碳源富集到的菌群结构进行分析。结果表明，利用2216E平板从以原油和正十六烷为碳源的富集液中各得到两株菌，分别为TJ-1、TJ-2和TJL-1和TJL-2，分子生物学方法鉴定结果表明，这4株菌分别为短小芽孢杆菌(Bacillus pumilus)、威尼斯不动杆菌(Acinetobacter venetianus)、Oceanobacillus Picturae和短小芽孢杆菌(Bacillus pumilus)。PCR-DGGE分析结果表明，以原油和正十六烷为碳源的富集液中优势菌分别有5种和2种，且2种富集液中的优势菌明显不同。对比PCR-DGGE和平板法分析结果，可以看出PCR-DGGE法能够提供更全面的菌群结构信息。
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| 关键词: 石油烃降解菌 PCR-DGGE 平板法 菌群分析 |
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| 基金项目:中国海洋大学与中海石油环保服务有限公司联合攻关项目（2006004） |
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| Analysis of hydrocarbon-degrading bacterium community structures in enrichment cultures with different carbon sources |
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YANG Shi-mei,ZHANG Yi-xiao,GAO Guang-jun,GUO Li-guo,LIANG Sheng-kang,SU Rong-guo
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| Abstract: |
| To enrich and isolate different function hydrocarbon-degrading bacteria from long-term oil-contaminated environment, crude oil and hexadecane were used as sole carbon sources respectively. On the other hand, bacterium community structures of enrichment were analysed by both plate method and PCR-DGGE (denaturing gradient gel electrophoresis) method. Two strains of bacteria, named as TJ-1 and TJ-2, were isolated from enrichment with crude oil as a sole carbon source. Strain TJL-1 and Strain TJL-2 were isolated with hexadecane as a sole carbon source. The 16SrDNA identified results showed that the four strains of TJ-1, TJ-2, TJL-1 and TJL-2 were Bacillus pumilu, Acinetobacter venetianus, Oceanobacillus Picturae, Bacillus pumilus, respectively. PCR-DGGE fingerprint showed that there were 5 dominating bacteria in crude oil culture and 2 dominating bacteria in Hexadecane culture. Dominating bacteria were different between crude oil culture and Hexadecane culture. It was indicated that PCR-DGGE method offers more comprehensive information of bacteria community structures than plate method.
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| Key words: Hydrocarbon-degrading bacterium PCR-DGGE plate method community analysis |