| 摘要: |
| 根据天津近海地理地貌特征设置了5个调查站位，分别采集不同水层的水样，进行了可培养细菌调查和多样性分析。调查结果表明，天津近海海水细菌总数为0.28×107～6.08×107 cfu/L。划线培养获得的菌株经16S rDNA扩增后，用RsaⅠ、MspⅠ限制性内切酶酶切，共获得27种酶切谱型。对27种谱型的细菌进行16S rDNA克隆和序列分析表明，天津近海海水可培养细菌分属于3个分类单元，即变形细菌(18种)、厚壁菌(5种)、拟杆菌(4种)。对天津近海海水可培养细菌的可能代谢类型进行了比较分析，为今后开发利用海洋生物资源和修复天津近海生态环境提供了数据。 |
| 关键词: 天津近海 可培养细菌 细菌多样性 16S rDNA RFLP分析 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金项目（30571421）; 国家科技支撑计划项目（2006BAD09A11） |
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| Preliminary investigation on the culturable bacteria diversity in Tianjin Coastal Waters |
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GUO Fei,GENG Xu-yun,LI Xiang,WEI Jun-li,SUN Jin-sheng
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| Abstract: |
| Tianjin is located on Bohai bay which is the largest inner ocean in China. Investigation on the bacteria diversity is of benefit to exploitting marine biological resources and improving the ecological environment of Tianjin coast. On the basis of the geographic features of Tianjin coast, 5 sites were selected to collect seawater samples for the examination of culturable bacteria and investigation of population diversity. Bacterial populations of seawater were enumerated by the plate count method. The results showed that plate count values ranged from 0.28×107 to 6.08×107 cfu/L. In order to understand the diversity of culturable bacteria, 276 strains of becteria were isolated and cultured from different layers in 5 sites. After PCR of 16S rDNA, 27 types of RFLP patterns were distinguished with RsaⅠ, MspⅠ. On the basis of the sequencing of 16S rDNA genes and comparing with the published sequences in GenBank database, 27 clonies fell into three groups: Proteobacteria, Irmicutes, Acteroidetes. Most of sequences were >96% identical to the published sequences. The Phylogenetic analysis has been performed by use of 16S rDNA sequencing and RFLP(restriction fragment length polymorphism). |
| Key words: Tianjin coast culturable bacteria diversity 16S rDNA RFLP analysis |
