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南冲绳海槽深海沉积物中度嗜盐菌系统进化及多样性分析
王静^1,2, 党宏月², 杨官品¹, 宋林生³
1.中国海洋大学海洋生命学院;2.中国科学院海洋研究所，海洋地质与环境重点实验室;3.中国科学院海洋研究所，实验海洋生物学重点实验室

摘要:

从南冲绳海槽MD05-2907站位的深海沉积物中分离到40株属于盐单胞菌科（Halomonadaceae）的中度嗜盐菌。耐盐度实验表明有12株最适生长盐度为30，19株为60，9株为100，多数菌在盐度高于150时生长速度迅速下降。有8株菌能产至少一种胞外蛋白酶。

关键词: 南冲绳海槽中度嗜盐菌盐单胞菌科(Halomonadaceae) 胞外蛋白酶

DOI：

分类号:

基金项目:中国科学院知识创新重要方向项目(KZCX2-YW-211-03，KZCX3-SW-233)；国家自然科学基金资助项目(40476058,40576069)；中国大洋协会国际海底区域研究开发“十一五”项目（受理号：020和126）

Physiochemical and phylogenetic analysis of moderately halophilic bacteria halomonadaceae in deep-sea sediments of the Southern Okinawa Trough

Abstract:

Forty strains of moderately halophilic Halomonadaceae bacteria were isolated from deep-sea sediments of the Southern Okinawa Trough station MD05-2907. An experiment of salt tolerance showed that 12 isolates grew optimally at salinity 30, 19 isolates at 60, and 9 isolates at 100. Growth rates of most isolates declined rapidly when the salinity was over 150. Extracellular hydrolytic enzyme screening showed that 8 isolates of Halomonadaceae were able to produce proteases.

Key words: Southern Okinawa Trough moderately halophilic bacteria halomonadaceae extracellular protease

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