引用本文:
【打印本页】   【下载PDF全文】   【View/Add Comment】  【Download reader】  【 Close 】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被：浏览 1685次   下载 1784次 本文二维码信息
码上扫一扫！
分享到： 微信 更多
字体:加大+|默认|缩小-
海水双壳类减数分裂器的一种免疫荧光显微观察方法
李永仁1, 阙华勇2, 张国范2
1.天津农学院水产科学系,天津市水产生态及养殖重点实验室;2.中国科学院海洋研究所
摘要:
为定性并定量研究海水双壳类受精过程中纺锤体和染色体的动态，改进了一种免疫荧光显微观察方法，包括样品的预处理、荧光染色和荧光显微观察。本方法适用于同步观测海水双壳类的纺锤体和染色体，具有操作简捷、清晰度高的优点。
关键词:  海水双壳类  减数分裂器  免疫荧光  显微观察术
DOI：
分类号:
基金项目:国家863计划资助项目（2006AA10A401）；国家自然科学基金资助项目（30471326）；山东省博士基金项目（2005BS07002）
An immune fluorescent method for observation of meiotic apparatus of marine bivalves
LI Yong-ren,QUE Hua-yong,ZHANG Guo-fan
Abstract:
An immunofluorescent method was developed to be adopted for observation of meiotic apparatus, which comprises chromosomes and the meiotic spindle, of fertilized eggs from marine bivalves. This method involves the pre-treatment of samples, fluorescent staining, and observation under fluorescence microscope. It is proved that this method is adequate for simultaneous observation of spindles and chromosomes in eggs with merits of simplicity in preparation and high definition of image of interest.
Key words:  marine bivalves  meiotic apparatus  immunofluorescence  microscopy
Copyright ©  Editorial Office for Marine Sciences Copyright©2008 All Rights Reserved
Supervised by: Chinese Academy of Sciences (CAS)   Sponsored by: Institute of Oceanology, CAS
Address: 7 Nanhai Road, Qingdao, China.  Postcode: 266071  Tel: 0532-82898755  E-mail: bjb@qdio.ac.cn
Technical support: Beijing E-Tiller Co.,Ltd.