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高磁性与无磁性趋磁细菌的获得及磁小体释放技术研究
张宇红1,2, 肖 天1
1.中国科学院海洋研究所海洋生态与环境科学重点实验室;2.中国科学院研究生院
摘要:
利用特殊的磁富集传代法获得高磁性和无磁性AMB-1菌株。通过透射电子显微镜(TEM)观察和Cmag值测定，对这两种菌株进行了验证。优选了条件，使高磁性AMB-1菌株在培养192 h后出现自溶现象，磁小体从细胞内释放出来。
关键词:  AMB-1  纳米磁小体  磁小体释放
DOI：
分类号:
基金项目:中国科学院海外杰出学者基金项目 (2003-1-5,2006-1-15)；中国科学院创新团队国际合作伙伴计划项目；人类前沿科学研究计划项目(HFSP, RGP0035/2004-C)
Cultivating technology of high-magnetic and non-magnetic AMB-1
ZHANG Yu-hong,XIAO Tian
Abstract:
Magnetospirillum sp. AMB-1 of high-magnetic and non-magnetic were obtained by special enrichment and inoculation method. After that this two kind of bacteria were validated by transmission electron microscope (TEM) and were measured Cmag. Furthermore, under optimizing growth conditions and cultivating time, AMB-1 of high-magnetic lysed and magnetosomes released from cells. As a result, magnetosomes were gotten conveniently by such method.
Key words:  AMB-1  nano-material  lysing
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