| 摘要: |
| 通过羟自由基清除作用、超氧自由基清除作用、还原能力和金属螯合能力等抗氧化活性实验, 测定了白色霞水母蛋白的抗氧化活性。实验结果表明: 白色霞水母蛋白具有较高的清除羟自由基的能力, 蛋白质量浓度为41μg/mL时, 清除率可达到85%; 白色霞水母蛋白对超氧阴离子的清除作用较弱, 蛋白质量浓度为77.7μg/mL时, 清除率<50% ; 白色霞水母蛋白有较强的还原能力, 但没有螯合能力; 白色霞水母蛋白的抗氧化活性受温度影响不大, 且巯基含量不是影响抗氧化活性的主要因素。 |
| 关键词: 白色霞水母(Cyanea nozakii Kishinouye) 蛋白 抗氧化活性 |
| DOI： |
| 分类号: |
| 基金项目:山东省中青年科学家科研奖励基金项目( 2006B??07003) |
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| Study on the antioxidant activity of the proteins from jellyfish Cyanea nozakii Kishinouye |
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刘 松
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| Abstract: |
| In this study, the antioxidant activity of proteins from jellyfish, Cyanea nozakii Kishinouye, was determined by various antioxidant assays, including hydroxyl radical-scavenging, superoxide anion radicalscav enging, reducing power and metal chelating activity. The proteins showed a strong scavenging activity on hydroxyl radical and the scavenging effect was 85% at the concent ration of 41μg/mL. However, the proteins had a low scavenging effect on superoxide anion radical, and the scavenging effect of the proteins didn't reach 50% at the concentration of 77.7μg/mL. The proteins showed a strong reducing power, but had no metal chelating activity. The radical scavenging activity was stable at a high temperature. The results showed that the hudro sulfide contained in CP wasn't the mainly factor on the antioxidant activity. |
| Key words: Cyanea nozakii Kishinouye protein antioxidant activity |
