| 摘要: |
| 为研制和生产鲈鱼苗疫苗提供依据,采用人工感染试验方法测试 了花鲈(Lateolabrax japonicus)烂尾、出血病病原菌W-1菌株(鳗弧菌Vibrio anguillarum)对花鲈苗的半致死浓度(LD50)及不同保存条件下的W-1菌株的致病力。试验结果表明:W-1菌浴感染花鲈苗的半致死浓度为 2.5×107.5个/mL,针刺后菌浴感染花鲈苗的半致死浓度为2.5×107个/mL;该病原菌为一条件致病菌,创伤感染是主要的致病途径;常温多次 传代、15℃、-20℃和-80℃等不同保存条件下W-1菌株的致病力均很强,但常温多次传代保存的菌株致病力比其他3种方法保存的菌株致病力稍差一些。 |
| 关键词: 花鲈(Lateolabrax japonicus)苗 鳗弧菌(Vibrio anguillarum) 人工感染 半致死浓度(LD50) |
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| 基金项目:教育部新世纪优秀人才支持计划项目(NCET-05-0597);国家863计划项目(2007AA09Z416);农业部公益性行业(农业)科研专项(nyhyzx07-046) |
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| Preliminary studies on the pathogenicity ofVibrio anguillarum W-1 in seaperch fry |
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| Abstract: |
| The lethal dose for 50%(LD50) of Vibrio anguillarum W-1 in seaperch fry and the pathogenicity of strain W-1 stored in different conditions were investigated in this paper. The results showed that the LD50 for seaperch fry with immersion infection was 2.5×107.5 cell/mL while the LD50 for the seaperch fry challenged with immersion after abdominal puncture was 2.5×107 cell/mL. The pathogen W-1 was an opportunistic pathogen and injury was the main infection factor. In addition, the strain W-1 reproduced many times as well as the strain W-1 stored at 15℃, -20℃ and-80℃ had a strong pathogenicity to the seaperch fry. |
| Key words: seaperch(Lateolabrax japonicus) fry Vibrio anguillarum artificial infection lethal dose for 50%(LD50) |
