| 摘要: |
| 研究了裙带菜(Undaria pinnatifida)配子体对除草剂草丁膦的敏感性以及温度对草丁膦处理效果的影响。结果表明,裙带菜配子体对草丁膦敏感;统计学分析得出了草丁膦在 不同温度条件下处理裙带菜配子体的半致死浓度(LD50)及95%可信限;温差达到10℃时,相同处理时间各组内不同处理温度样品的LD50之间呈现显著 差异,第6天的LD50由10℃组的15.78 mg/L降为20℃组的11.56 mg/L和25℃组的10.33 mg/L。研究发现可以尝试采用草丁膦作为裙带菜配子体遗传转化的选择试剂。 |
| 关键词: 裙带菜(Undaria pinnatifida) 配子体 温度 草丁膦(phosphiothricin,PPT) 选择标记 |
| DOI： |
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| 基金项目:中国科学院知识创新工程资助项目(KZCX3-SW-215) |
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| Study on selective marker for genetic engineering of Undaria pinnatifida gametophytes |
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| Abstract: |
| The sensitivity of Undaria pinnatifida gametophytes to PPT(phosphiothricin) at different temperatures(10.0, 15.0, 20.0, 25.0℃, ±0.5℃) was tested. The results of LD50 and 95% Confidence Range of PPT showed that U. pinnatifida gametophytes were sensitive to PPT; increasing the cultivation temperature could significantly decrease the LD50 of PPT. At the sixth day, the LD50 dropped from 15.78 mg/L at 10.0℃ to 11.56 mg/L at 20.0℃ and 10.33 mg/L at 25.0℃. The results mentioned above indicated that PPT could be a promising selective pressure for U. pinnatifida gametophytes transgenic research. |
| Key words: Undaria pinnatifida gametophytes temperature phosphiothricin(PPT) selective marker |
