| 摘要: |
| 海洋来源放线菌H2003发酵产物的粗提物对小鼠乳腺癌细胞 tsFT210具有明显的细胞毒活性。为了发现其抗肿瘤活性成分,采用活性跟踪的分离方法,经多次硅胶柱色谱、凝胶柱色谱及高效液相色谱分离,从菌株 H2003发酵液中分离得到了7个环二肽化合物。经核磁共振光谱、质谱和旋光分析,其结构分别鉴定为cyclo(L-Leu-L-Pro) (1),cyclo(D-Val-L-Pro)(2),cyclo(Gly-L-Leu)(3),cyclo(L-Pro-L-Try) (4),cyclo(Gly-D-Phe)(5),cyclo(Gly-L-Pro)(6)和cyclo(L-Ala-L-Phe)(7)。其中化合物1 通过SRB法测试表现出弱的抗肿瘤活性,系首次报道。 |
| 关键词: 海洋放线菌 次级代谢产物 环二肽 抗肿瘤活性 |
| DOI： |
| 分类号: |
| 基金项目:国家863计划项目(2003AA624020);山东省科技攻关项目(012110107);山东省重点基金项目(Z2001C01);国家教育部长江学者奖励计划项目;青岛市自然科学基金项目(04-2-JZ-81) |
|
| Structural identification and anti-tumor activity of Diketopi-perazines from secondary metabolites of marine-derived actinomycete H2003 |
|
|
| Abstract: |
| The fermentation of the marine-derived actinomycete H2003 showed toxicity on tsFT210 cells. In order to isolate and identify the bioactive components, the secondary metabolite of the fermentation of H2003 was studied. Through bioassay-guided fractionation of necrosis on tsFT210 cells, seven diketopiperazines were isolated from the secondary metabolites of actinomycete H2003 by repeating chromatography over silica gel, sephadex LH-20 and HPLC. Their structures were identified by the spectroscopic methods and anti-tumor activities were assayed by SRB method. Compound(1) showed weak anti-tumor activity on tsFT210 cells. |
| Key words: marine-derived actinomycete secondary metabolite diketopiperazine anti-tumor activity |
