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养殖杂色鲍暴发病超微病理学研究
王江勇1,2, 郭志勋1, 冯 娟1, 刘广锋1, 徐力文1, 陈毕生1, 潘金培2
1.中国水产科学研究院 南海水产研究所;2.中国科学院 南海海洋研究所
摘要:
从1999年至2002年在中国南方沿海许多养殖场的杂色鲍 (Haliotis diversicolor Reeve)出现了严重的疾病,该病可感染各种规格的鲍,死亡很快,死亡率高。病鲍的外观症状表现为外套膜收缩,腹足表面变黑,肌肉变得僵硬。通过电镜观 察,在肝胰腺、外套膜、肾、鳃及肠等组织中发现了一种球状病毒。病毒粒子大小为100~130nm。该病毒一般存在于间质细胞的细胞质中,为双层质膜所包 裹。染病鲍细胞器如滑面内质网扩张、线粒体崩解,糖原粒、核糖体减少,核膜松疏、溶解、消失,核质边缘化。
关键词:  病毒  病原  病理学  杂色鲍(Haliotis diversicolor Reeve)  
DOI:
分类号:
基金项目:广东省重大科技攻关资助项目(2KB05301N)
Studies on ultrastructural pathology of outbreaking disease of Haliotis diversicolor Reeve
Abstract:
A serious disease had occurred in many commercial factories of Haliotis diversicolor Reeve during 1999 and 2002 in coastal areas of the South China Sea. It was infectious to all sizes of abalones with high mortality. The symptoms of the sick abalone were as follows: the mantle shrunk, the muscle became stiff and the surface of pleopod blacked. Under electron microscope, a kind of spherical virus was detected in the tissues of digestive gland, mantle, gill and intestine. The diameter of the virion was about 100~130nm, it was observed in the cytoplasm with double membranes. The organelle such as endoplasmic reticulum expanded, mitochondria dilated, the number of ribosome reduced, nuclear membranes dissolved or disappeared, the edges of nucleolus swelled. The pathogenicity of the virus was confirmed by an experimental infection with healthy abalones.
Key words:  virus  pathogen  pathology  Haliotis diversicolor Reeve  
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