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用改进的超声波-溶菌酶法制备钝顶螺旋藻原生质球
银红梅1, 张学成1, 王志刚1
中国海洋大学生命科学与技术学部
摘要:
用改进的超声波-溶菌酶法制备钝顶螺旋藻原生质球。钝顶螺旋 藻(Spirulina platensis)经超声波破碎后,用1.5 mol/L NaCl的Zarrouk培养基洗去细胞外壁的胶质鞘,然后进行酶解。酶解条件为: pH7.2的0.2 mol/L磷酸钾缓冲液,0.8 mol/L KCl,0.5%溶菌酶,28～30℃水浴震荡酶解5～7 h,获得40%以上有活力的钝顶螺旋藻原生质球。
关键词:  钝顶螺旋藻(Spirulina platensis)  原生质球  超声波  溶菌酶  
DOI：
分类号:
基金项目:
Preparation of spheroplasts from Spirulina platensis by ultrasonic-lysozyme method
Abstract:
The obtainment of spheroplasts of Spirulina platensis by the improved ultrasonic-lysozyme method was dealt with in the paper. S. platensis was broken by ultrasonic, and the gelatinous sheath of the cells was rinsed with Zarrouk medium containing 1.5 mol/L NaCl. Then the cells were digested by lysozyme at 28～30℃ for 5～7 hours, the enzyme solution was composed of 0.8 mol/L KCl, 0.5% lysozyme and 7.2 pH, 0.2 mol/L buffer of K2HPO4 and KH2PO4. Results showed that spheroplasts rate could be obtained above 40%.
Key words:  Spirulina platensis  spheroplasts  ultrasonic  lysozyme  
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