| 摘要: |
| 用高效液相色谱法研究静注、肌注和口服氟苯尼考在中国明对虾 (Fenneropenaeus chinensis)体内的药代动力学特征,所得数据用MCP-KP程序分析。结果表明,3种给药方式的血药经时过程均符合二室开放模型,符合一级吸收二项指数方程,血窦注射、肌肉注射、口服药饵的时间对药物质量浓度的理论方程分别是:C血注=16.38e- 2.58t+0.32e-0.1t,C肌注=18.7e-2.57t+0.26e-0.12t,C药饵=15.08e–1.49t+0.65e- 0.09t–15.74e–10.38t。主要药物代谢动力学参数分别是:吸收半衰期(t(1/2)α)为0.269,0.270和0.465h,消除半 衰期(t(1/2)β)为6.921,6.494和7.903h,表观分布容积(Vβ)为1.287,1.293和2.421L/kg,总体清除率 (Cs)为0.129,0.138和0.213L/(h·kg),药时曲线下总面积(Au,c)为0.755,0.681和0.666L/(h·kg)。 肌肉注射和口服给药的生物利用度(F)分别是90.20%和97.58%。 |
| 关键词: 氟苯尼考 中国明对虾(Fenneropenaeus chinensis) 药代动力学 高效液相色谱(HPLC) |
| DOI： |
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| 基金项目:国家科技攻关计划资助项目(2002BA906A39);国家农业科技跨越计划资助项目(2003-5);山东省科技发展计划资助项目(2003-4-1) |
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| Single dose pharmacokinetics of florfenicol in Fenneropenaeus chinensis |
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| Abstract: |
| The pharmacokinetics of intravenously, intramuscularly and orally administered florfenicol were determined in Fenneropenaeus chinensis. The study was performed at 19～22 ℃. A dose of 15mg/ kg body mass was administered either intravenously or intramuscularly. The oral dose was13.56 mg/kg body mass. At 8～10 time points, from 0.083 h to 24 h after administration, blood was sampled from 8 individual shrimps in each group. The plasma was assayed for florfenicol using an HPLC method. The pharmacokinetic modling of the results was performed using the computer program MCP-KP. Following the three administrations, the plasma concentration–time data of florfenicol were all best described by two-compartment open model. The important parameters of intravenous, intramuscular and oral administrations were listed. The volumes of distribution( Vβ) were 1.287, 1.293, 2.421 L/kg, the total body clearance(Cs) was 0.129, 0.138, 0.213 L/(h·kg), the elimination halflives (t(1/2)β were 5.91, 7.57, 8.01 h, the peak plasma concentrations(Cmax) were 6.970, 6.396, 2.772 mg/L, and were estimated to occur at 0.206, 0.239, 0.605 h (Tmax) following dosing. The bioavailabilities (F) of intramuscular and oral administrations were 90.20% and 97.58%. |
| Key words: florfenicol Fenneropenaeus chinensis pharmacokinetics high performance liquid chromatography(HPLC) |
