引用本文:
【打印本页】   【下载PDF全文】   【View/Add Comment】  【Download reader】  【 Close 】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被：浏览 1426次   下载 1916次 本文二维码信息
码上扫一扫！
分享到： 微信 更多
字体:加大+|默认|缩小-
不结球白菜叶片壳聚糖酶的理化特性研究
陈惠萍1,2, 徐朗莱1
1.南京农业大学生命科学学院;2.华南热带农业大学农学院
摘要:
通过不结球白菜叶片壳聚糖酶对壳聚糖的降解,研究了温度、 pH、时间及金属离子等对此壳聚糖酶的影响。结果表明,壳聚糖酶的最适温度为45℃,最适pH为5.5,酶促反应速度在6 h内达到最大值;Mn2+和Cu2+对壳聚糖酶促反应分别起到促进和抑制的作用。经薄板层析分析证实,壳聚糖酶降解壳聚糖为低聚物。
关键词:  不结球白菜  壳聚糖酶  最适温度  最适pH  低壳聚糖
DOI：
分类号:
基金项目:
Studies on chemical and physical characters of chitosanase from non-heading Chinese cabbage leaves
Abstract:
The effects of temperature, pH, time and metal ion on chitosanase activity from non-heading Chinese cabbage (Brassica campestris L.ssp.chinensis(L.) Makino) leaves were investigated. The results show that the optimal temperature and pH are 45℃ and 5.5 in degrading chitosan respectively. Otherwise, (analysis) of thin-board chromatograph revealed that chtiosan was decomposed into chitooligo saccharedes by chitosanase.
Key words:  non-heading Chinese cabbage  chitosanase  optimal temperature  optimal pH  chitooligosaccharides
Copyright ©  Editorial Office for Marine Sciences Copyright©2008 All Rights Reserved
Supervised by: Chinese Academy of Sciences (CAS)   Sponsored by: Institute of Oceanology, CAS
Address: 7 Nanhai Road, Qingdao, China.  Postcode: 266071  Tel: 0532-82898755  E-mail: bjb@qdio.ac.cn
Technical support: Beijing E-Tiller Co.,Ltd.