| 摘要: |
| 用生理生化的方法研究了UV-B辐射对海洋微藻———小新月 菱形藻(Nitzschia closterium)生理生化的影响。结果表明:(1)随着UV-B暴露时间的延长,UV-B辐射对藻的生长以及光合速率有抑制作用,并且时间越长, 抑制作用越明显;(2)UV-B辐射使小新月菱形藻可溶性糖含量降低,而且在最初的2d,UV-B辐射使可溶性糖含量降低迅速,第4天的可溶性糖含量稍有所升高但与第2天相比差异不显著,UV-B辐射第6天,可溶性糖含量又迅速降低;(3)UV-B辐射的可溶性蛋白含量在第2天明显升高,随后又快速下降; (4)对照组的还原型谷胱甘肽(GSH)含量的变化随时间延长而升高,UV-B辐射第2天的GSH含量比初始状态(0d)的GSH含量稍有升高,但变化不明显,第2天之后,GSH含量随时间延长而快速降低;(5)对照组的过氧化物酶(POD)活性随时间延长而升高,UV-B辐射第2天的POD活性比初始状态(0d)的POD活性显著升高,第2天之后,POD活性随时间延长而快速降低。 |
| 关键词: UV-B辐射 海洋微藻 生理生化响应 |
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| 基金项目:国家自然科学基金资助项目(30270258);山东省优秀中青年奖励基金资助项目(03BS120) |
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| The physiological and biochemical responses of marine microalgae to UV-B radiation |
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| Abstract: |
| The physiological and biochemical responses of marine microalgae to UV-B irradiation were examined by using the physiological and biochemical methods in this article. The results showed that（1） UV-B irradiation can inhibit the growth and photosynthetic rate of Nitzschia closterium, and the inhibiting effects were more significant when the time was prolonged;（2） UV-B irradiation decreased soluble sugar. In the initial 2 days, soluble sugar of N. closterium decreased rapidly, the soluble sugar on the 4th day has no major change compared with the soluble sugar on the 2nd day, on the 6th day, the soluble sugar decreased again;（3）The protein content of UV-B treated group significantly increased on the 2nd day, rapidly decreased subsequently;（4）GSH content of control group increased as time prolonged, the GSH content on the 2nd day has no major change with initial time（0 d）, after the 2nd day, the GSH content decreased rapidly;（5） POD activity of control group increased as time prolonged, the POD activity on the 2nd day increased significantly compared with POD activity on the initial time（0 d）, after the 2nd day, POD activity rapidly decreased as time prolonged. |
| Key words: UV-B radiation marine microalgae physiological and biochemical responses |
