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| 漠斑牙鲆养殖群体RAPD遗传多样性的初步分析 |
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尤 锋1, 吴志昊1, 王 伟1,2, 徐冬冬1, 许建和1,3, 倪 静1, 孙 威1, 张培军1, 徐永立1, 杨学宋4, 孙寿科4
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1.中国科学院海洋研究所;2.中国海洋大学生命科学与技术学部;3.淮海工学院;4.莱州大华水产有限公司
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| 摘要: |
| 利用RAPD技术对由美国引进的漠斑牙鲆(Paralichthys lethostigma)养殖子一代群体的遗传多样性进行了研究。实验用漠斑牙鲆鱼苗于2004年10月取自山东莱州大华水产有限公司,共24尾,全长平 均为16.6 mm±1.99 mm。活体取其肌肉,用高盐法抽提获得总DNA,琼脂糖电泳检测,Beckman DU-6100型紫外分光光度仪定量后用随机引物进行PCR扩增,从25个随机引物中筛选出11个用于群体遗传学分析,共获得77条清晰重复性高的DNA 片段,大小在100～2 000 bp之间,多态谱带比例和群体平均杂合度分别为66.23%和0.352 6。 |
| 关键词: 漠斑牙鲆(Paralichthys lethostigma) 养殖群体 RAPD 遗传多样性 |
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| 基金项目:国家高技术研究发展(863)计划探索课题(2002AA629160);国家自然科学基金资助项目(30271036);山东省自然科学基金资助项目;山东省科学技术发展计划(2005GG3205071) |
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| Primary analysis on genetic diversity in cultured stock of Paralichthys lethostigma |
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| Abstract: |
| The genetic diversity of a cultured stock in Paralichthys lethostigma which the parental stock introduced from America was studied as revealed by RAPD markers. Fish samples(24 individuals) were collected from Dahua Fishing Limited Company of Laizhou in October, 2004. Their total DNA was extracted from muscle tissue of sample using high salty method. The main results show as follows: 11 from 25 random primers were selected to use for stock experiment. Seventy-seven clear repeatable RAPD bands ranged from 100 to(2 000) bp were recorded separately, and an average of 7 bands was gained by per primer. The mean percentage of polymorphic loci(P) of this stock was 66.23% and the average heterozygosity(H) of it was(0.352 6.) |
| Key words: Paralichthys lethostigma cultured stock RAPD genetic diversity |