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烟曲霉产壳聚糖酶液体发酵条件研究
周桂¹, 何子平², 禤金彩¹
1.广西民族大学化学与生态工程学院;2.广西农业科学院

摘要:

以烟曲霉(Aspergillus fumigatus)为出发菌,通过选择适合的培养条件,筛选出产壳聚糖酶的菌株。实验结果表明,烟曲霉是良好的产壳聚糖酶出发菌。烟曲霉液体发酵产壳聚糖酶最适培养基组成为:壳聚糖1%, KH₂PO₁ 0.06%,尿素0.2%,(NH₄)₂SO₄ 0.1%,MgSO₄ 0.012%, 20%土豆汁 3%,NaCl 0.05%。在pH 5.0、温度28℃、摇床转速120 r/min培养条件下振荡培养3 d,烟曲霉产壳聚糖酶的能力最强,达25.1 U/mL。

关键词: 烟曲霉(Aspergillus fumigatus) 壳聚糖酶液体发酵

DOI：

分类号:

基金项目:广西壮族自治区青年基金项目(桂科青0481036);广西壮族自治区教育厅资助项目(桂教科研2002-316)

Study on conditions of chitosanase from Aspergillus fumigatus by liquid fermentation

Abstract:

A mutant of Aspergillus fumigatus, which could synthesize chitosanase effectively under the optimized conditions, was obtained with selective nutrient mediums. The optimized liquid fermentation mediums were as follows: chitosan 1%, KH₂PO₄ 0.06%, Urea 0.2%, (NH₄)₂SO₄ 0.1%, MgSO₄ 0.012%, 20% potato juice 3%, NaCl 0.05%, and pH 5.0. After 3 days’ fermentation at 120 r/min and 28℃, the chitosanase activity produced by the mutant of A. fumigatus reached a higher level of 25.1 U/mL.

Key words: Aspergillus fumigatus chitosanase liquid fermentation

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