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球等鞭金藻3011浓缩细胞低温保存技术的研究
阎斌伦1, 朱明1, 张学成2
1.淮海工学院江苏省海洋生物技术重点建设实验室;2.中国海洋大学生命学院
摘要:
为了探讨球等鞭金藻3011(Isochrysis galbana Parke)浓缩细胞的保存条件,通过研究其在不同温度条件下的长期保存效果来确定最佳的保存温度范围。实验结果表明,在0～10℃的低温下长期(2个月以上)保存后,球等鞭金藻3011获得较高的存活率(70%以上),并且在恢复生长时保持较高的比生长率,因此0～10℃是球等鞭金藻3011浓缩细胞长期保存的最佳温度范围。
关键词:  球等鞭金藻3011(Isochrysis galbana Parke)  浓缩细胞  保存  温度  存活率  生长率
DOI：
分类号:
基金项目:江苏高校高新技术产业发展资助项目(JH B04-020)
Study on the preservation technique for Isochrysis galbana Parke condensed at low temperature
Abstract:
In this paper, effects of different temperatures on Isochrysis galbana Parke condensed after long-term preservation (more than 2 months) were investigated, the results showed that cells reached high survival rate(more than 70%) after being conserved at temperature 0～10℃, and kept high growth rate after recovering. So 0～10℃ could be considered as the most optimum range of temperature for preserving this kind of alga.
Key words:  Isochrysis galbana Parke  condensed cells  preservation  temperature  survival rates  growth rate
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