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小球藻提取物的生物活性研究
刘海琴1, 韩士群1
江苏省农科院农业资源与环境研究中心
摘要:
研究了小球藻(Chlorella pyenoidosa)酶解提取物(CGF)的主要成分、稳定性及其增强小鼠免疫调节的生理活性功能。CGF中蛋白质、氨基酸、核苷酸、多糖、维生素分别达到56.6%,42.59%,6.8%,4.8%,1.7%。采用75,150,450 mg/(kg·d)剂量经30 d对小鼠喂食,进行免疫调节试验,结果表明,150、450 mg/(kg·d)剂量的小球藻生长因子可以明显增强二硝基氟苯(DNFB)引起的小鼠迟发性超敏反应,对造血功能受损的小鼠有恢复作用, 以及增强小鼠单核-巨噬细胞吞噬功能的作用。实验表明服用CGF可以增强免疫能力。
关键词:  小球藻(Chlorella pyenoidosa)  提取物  免疫
DOI:
分类号:
基金项目:江苏省高新技术项目(BG2003321)
Physiological function of Chlorella growth factor
Abstract:
The main components and stability of Chlorella Growth Factor (CGF) which was extracted from cells of Chlorella with enzyme were analyzed. Meanwhile, its function of stimulating immunity on mice was investgated. CGF contained 56.6% protein, 42.59% amino acid, 6.8% nucleoside acid, 4.8% polysaccharide and 1.7% vitamin. The mice was fed with CGF at the rates of 75,150 and 450mg/kg·d in the experiment of stimulating immunity function. The results showed that applying CGF at the rates of 150 and 450 mg/kg·d could increase allergic reaction induced by Dinitrofuorobenzene (DNFB), enhance the proliferation of immunoinhibited murine splenocytes, and stimulate phagocytic function by macrophages. In general, CGF can enhance immunity function.
Key words:  Chlorella pyenoidosa, extract, immunity function
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