| 摘要: |
| 利用RAPD技术首次对中国海南岛环岛的大珠母贝(Pinctada maxima Jameson)遗传多性进行分析。从20条10bp引物中选取7条引物用于群体遗传多样性分析,共检测出22个位点,其中11个位点(占50%)显多态性,Shannon多样性值为0.117。用非加权配对算数平均法(UPGMA)聚类分析的结果:12个体间遗传相似系数最大为1.000,最小为0.706,平均遗传相似系数为0.8438。表明海南岛大珠母贝群体间遗传多样性不一,遗传分化较大,因此很有必要对遗传多样性水平较高的种群,划定保护范围加以重点保护,以扩大和恢复大珠母贝的种质资源。 |
| 关键词: 大珠母贝[Pinctada maxima (Jameson)] RAPD 遗传多样性 |
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| 基金项目:国家科技基础性工作项目（2000DEA30014） |
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| Genetic diversity of Pinctada maxima (Jameson) germplasm by RAPD analysis |
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| Abstract: |
| Genetic diversity of Pinctada maxima germplasm from Hainan Islands was studied by RAPD for the first time. Among total 20 10pb primers, 7 were selected for group genetic diversity research. 22 DNA fragments were recognized, in which 11 were polymorphic. The Shannnon Index of diversity of P. maxima population was 0.1172. The genetic similarities varied between 1 and 0.706, indicating that the genetic diversities of P. maxima inside the population were obviously different from each other. In order to resume the quality of P. maxima strain, measures must be taken to protect population of P. maxima that are more polymorphic. |
| Key words: Pinctada maxima (Jameson) RAPD genetic diversity |
