| 摘要: |
| 基于银因其具有良好的广谱抗菌活性和低毒性而在医学方面有着较为广泛的应用,对银离子(硝酸银)和含银不锈钢的抗菌活性进行了初步研究。实验结果表明,在106CFU/mL的初始接种浓度下,银离子对枯草杆菌、大肠杆菌和假单胞菌的最小抑菌质量浓度和最小杀菌质量浓度分别为2,6,8mg/L和2,8,10mg/L。大肠杆菌经8mg/L的银离子处理2h后,有99.9%以上的细菌被破坏。含银不锈钢也表现了较强的抗菌性能。在普通的、银合金和银涂层的SUS304不锈钢表面附着的单层生物膜浸泡在PBS溶液中3d后,经CFDA-DAPI双染色和荧光显微镜观察计数获得的假单胞菌的平均存活率为98.0%,38.5%和15.1%。 |
| 关键词: 抗菌活性 银离子 含银不锈钢 |
| DOI： |
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| Antibacterial property of silver ions and silver-containing stainless steels |
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| Abstract: |
| It is well known that silver has good antiseptic properties and has been used widely in medical treatment. In the research, antibacterial property of silver ion and silver-containing stainless steel were studied to develop silver-additive antibacterial stainless steels application. The results showed at inoculum size of 106 CFU/mL, the minimum inhibitory concentration and minimum bactericidal concentration of silver ion were 2, 6, 8 and 2, 8, 10 μg/mL to Bacillus subtilis IAM 1069, Escherichia coli K12 and Pseudomons aeruginosa PAO1, respectively. Viable bacteria of Escherichia coli K12 was lower than 0.01% after exposed to 8 μg/mL silver ion concentration for 2 h. The survival of Pseudomons aeruginosa PAO1 on the surfaces of regular stells, silver-alloyed and silver-coated stainless steels were in average level of 98.0%, 38.5% and 15.1% after being exposed to PBS for 3 days, respectively, revealed by direct epifluorescence enumeration method and ouble stained technology of CFDA and DAPI. |
| Key words: antibacterial property silver ion silver-containing stainless steels |
