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三丁基锡(TBT)对牡蛎细胞基因组DNA影响的RAPD分析
韩雅莉1, 管云雁2, 李兴暖2
1.广东工业大学轻工化工学院生物工程系;2.汕头大学理学院生物系
摘要:
以僧帽牡蛎(Saccostrea cucullata)为材料,取其鳃进行组织培养,同时对其进行7个浓度0,0.02,0.2,2,20,200,2000μg/L的三丁基锡(TBT)处理,提取鳃细胞基因组DNA。从38个随机引物中筛选出4个引物,对上述DNA样品进行利用RAPD-PCR扩增分析。结果表明,TBT处理的基因组DNA与对照组样品比较有一定差异,扩增条带总数均有所减少,随TBT处理浓度的不同,条带缺失或者条带深浅变化呈明显差异性,表明不同浓度TBT处理会对DNA造成不同程度的损伤。
关键词:  僧帽牡蛎(Saccostrea cucullata)  组织培养  RAPD  三丁基锡(TBT)
DOI：
分类号:
基金项目:国家自然科学基金(30271033);广东省科技兴海重大项目(A200005F02)
Study on the effects of TBT on genome DNA of oyster(Saccostrea cucullata)by RAPD
Abstract:
The effects of Tributyltin on genomic DNA of oyster (Saccostrea cucullata) gill cell that was cultured and treated by 0, 0.02, 0.2, 2, 20, 200 and 2000 μg/L concentration of TBT was studied by RAPD-PCR method. The genomic DNA of the gill cell was extracted and 4 primers was chosen among 38 primers. Results showed that amplified products have some difference between control (from normal individual cells) and groups treated by TBT. The bands of DNA had decreased with increasing concentration of TBT. The results suggested that TBT would destroy genome DNA of oyster cell in different level.
Key words:  Saccostrea cucullata  tissue culture  RAPD  TBT
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