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海洋发光细菌快速测定四环素效价的方法研究
朱文杰1, 杨颐康1
华东师范大学生物学系
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A QUICK ASSAY FOR TETRACYCLINE
Abstract:
An anti-luminescent assay of Tetracycline that, like many antibiotic substances, quenches the light of marine form luminous bacteria effectively was studied with Photobacterium phosphoreum A2 as an indicator. Good results were obtained in tests with luminous bacterial concentration ca. 5×107 cells/ml. Light intensity was determined by photomultiplier (GDBIOM) of photoluminometer. Experimental data were expressed as voltage or a percentage of a control. Within the range of 30 μ/ml to 140μ/ml, the curves of tetracycline concentration plotted against light intensity gave good linearity. The correlation coefficient of the curves obtained were -0.996, -0.978, -0.988. The tests could be done within an hour. So, comparing with cylinder plate assay, this one is faster and simpler.
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