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毛蚶体内B₁₂的提取和B₁₂中⁶⁰Co的测定
杨嘉东¹
国家海洋局第三海洋研究所

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EXTRACTION OF VITAMIN B₁₂ FROM ARCA SUBCRENATA LISCHKE AND EFFECT OF ⁶⁰Co ON B₁₂ CONTENT

Abstract:

This paper deals with the extraction procedures of vitamin B₁₂ from Area Subcrenata Lischke and effect of ⁶⁰Co on B₁₂ content. One group of animals were maintained in a pool of sea water with ⁶⁰CoCl₂ (radioactivity of ⁶⁰Co is 5×10^-8Ci/1), another was cultured without ⁶⁰CoCl₂, and those collected from the sea were used as control. The amount of B₁₂ was measured when the biological equilibrium of the uptake of ⁶⁰CoCl₂ was reached. The result shows that the amount of B₁₂ in the animals kept in the pool with ⁶⁰CoCl₂ is 0.294μg per gram (fresh weight), the second being 0.142μg, whereas the control being 0.239μg, which implies that the animals under low level but long term gamma ray irradiation may enhance the synthesis of B₁₂.

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