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引用本文:王静,臧帆,秦松.螺旋藻藻蓝蛋白对酒精性肝损伤大鼠肠道菌群的影响[J].海洋科学,2023,47(12):93-101.
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螺旋藻藻蓝蛋白对酒精性肝损伤大鼠肠道菌群的影响
王静1,2, 臧帆2, 秦松1,2
1.滨州医学院药学院, 山东 烟台 264003;2.中国科学院烟台海岸带研究所, 山东 烟台 264003
摘要:
酒精性肝病(alcoholic liver disease, ALD)是中国常见的肝脏疾病之一, 严重危害人民健康。目前, 缺乏治疗酒精性肝病的有效方法。螺旋藻藻蓝蛋白(phycocyanin, PC)是一种天然蛋白质, 具有抗炎、抗氧化等作用。本文研究了PC对大鼠酒精性肝损伤的保护作用及其对肠道菌群的影响。利用酒精诱导酒精性肝损伤模型, 将大鼠随机分为4组, 分别为对照组, 模型组, 阳性对照组, 藻蓝蛋白组, 灌胃PC干预之后, 通过肝脏切片的Masson和HE染色的病理分析判断肝损伤的程度, 之后通过16S rRNA高通量测序技术检测大鼠肠道菌群的组成和结构。模型组大鼠肠道菌群发生了紊乱, 显著降低了双歧杆菌(Bifidobacteriaceae)的丰度, 该菌种可以减少肝脏炎症和肝酶, 口服PC后Bifidobacteriaceae的丰度显著增加。此外, 酒精诱导后, 乳酸杆菌(Lactobacillaceae)的丰度略微增加, 促进酒精代谢。藻蓝蛋白能够通过改变酒精性肝损伤大鼠的肠道菌群构成, 从而起到缓解酒精诱导的肝损伤的效果。
关键词:  酒精性肝损伤  藻蓝蛋白  肠道菌群
DOI:10.11759/hykx20221028001
分类号:R9
基金项目:吉林省与中国科学院科技合作高技术产业化专项资金项目(2023SYHZ0040)
Effect of Spirulina phycocyanin on intestinal flora of rats with alcoholic liver injury
Abstract:
Alcoholic liver disease (ALD) is one of the most common liver diseases in China and is a serious health risk to people’s health. Currently, effective methods to treat ALD are lacking. Spirulina phycocyanin (PC) is a naturally occurring protein with anti-inflammatory and antioxidant properties. In this study, we investigated the protective effect of PC on the intestinal flora of rats with alcoholic liver injury. Using an alcohol-induced alcoholic liver injury model, rats were randomly divided into four groups: control group, model group, positive control group, and PC group. After gavage of PC intervention, pathological analysis of the extent of liver injury was determined using Masson and hematoxylin and eosin staining of liver sections, followed by detection of the composition and structure of rat intestinal flora using 16S rRNA high-throughput sequencing technology. The intestinal flora of rats in the model group was disrupted, significantly reducing the abundance of Bifidobacteria, a species that reduces liver inflammation and liver enzymes; however, after oral administration of PC, the abundance of Bifidobacteria significantly increased. Furthermore, the abundance of Lactobacillus slightly increased following alcohol induction, thereby promoting alcohol metabolism. PC was able to alleviate alcohol-induced liver injury in rats by altering the composition of intestinal flora.
Key words:  alcoholic liver injury  phycocyanin  intestinal flora
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