| 引用本文: | 房莹莹,陈璐璐,刘松涛,孙飞,吴燕玲,李鑫,刘鹰,马贺.光谱对许氏平鲉消化代谢节律及生理应激研究[J].海洋科学,2023,47(10):94-111. |
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| 光谱对许氏平鲉消化代谢节律及生理应激研究 |
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房莹莹1,2, 陈璐璐1,2, 刘松涛1,2, 孙飞1,2, 吴燕玲1,2, 李鑫1,2, 刘鹰1,3, 马贺1,2
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1.大连海洋大学海洋科技与环境学院, 辽宁 大连 116023;2.设施渔业教育部重点实验室, 辽宁 大连 116023;3.浙江大学生物系统工程与食品科学学院, 浙江 杭州 310058
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| 摘要: |
| 为探究不同光谱下许氏平鲉的消化代谢水平及外周激素节律,在5种光谱(红光、绿光、黄光、蓝光、白光,光周期12L∶12D)下,通过对许氏平鲉的血清、肠道、肝脏进行24 h (8:00,12:00,16:00,20:00,24:00)取样,结果显示:不同的光谱下许氏平鲉的消化代谢酶活性除胰蛋白酶(TRY)外均具有节律性,红光、黄光下α-淀粉酶(α-AMS)、脂肪酶(LPS)活性的峰值相位与白光组相比左移;胰蛋白酶活性无时间差异性;在绿光、黄光及红光下乳酸脱氢酶(LDH)活性的峰值相位与白光组相比发生左移,在五种光谱环境中,红、黄光影响消化酶活性峰值提前出现;丙酮酸激酶(PK)活性在绿、蓝及红光下峰值相位左移;己糖激酶(HK)的活性在绿、蓝、黄光下,其峰值相位与白光组相比发生左移,在五种光谱环境中,绿、蓝光影响代谢酶活性类峰值提前出现。许氏平鲉血清中褪黑素含量呈现昼低夜高分泌水平;皮质醇的含量表现为在白天降低,午夜显著升高至最高值后又持续降低。峰值相位的改变预示着生理节律的变动,以上结果表明不同光谱可以影响鱼类的生理代谢节律,在今后养殖过程中需要充分考虑光谱对养殖生物的生物学作用,进而制定更为合理的光照条件。 |
| 关键词: 许氏平鲉 光谱 昼夜节律 消化代谢 应激 |
| DOI：10.11759/hykx20220819003 |
| 分类号:S9645.3 |
| 基金项目:国家自然基金(32202961);辽宁省科学技术计划项目(2021JH2/10200011);设施渔业教育重点实验开放课题(202202);现代农业产业技术体系专项资金(CARS-49) |
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| Effects of spectra on digestion, metabolic rhythm, and physiological stress of Sebastes schlegelii |
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FANG Ying-ying1,2, CHEN Lu-lu1,2, LIU Song-tao1,2, SUN Fei1,2, WU Yan-ling1,2, LI Xin1,2, LIU Ying1,3, MA He1,2
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1.College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China;2.Key Laboratory of Environment Controlled Aquaculture(KLECA), Dalian 116023, China;3.College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
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| Abstract: |
| The serum, intestinal tract, and liver of Sebastes schlegelii were sampled over a period of 24 h (8: 00, 12: 00, 16: 00, 20: 00, and 24: 00) under five spectra (red, green, yellow, blue, and white lights, respectively; photoperiod 12L: 12D). to investigate the digestion and metabolism levels and peripheral hormone rhythms. Results revealed that the digestion and metabolism enzymes of Sebastes schlegelii under different spectra exhibited rhythmic patterns, except for trypsin. The peak phases of α-amylase (α-AMS) and lipase (LPS) under yellow light shifted to the left compared to those under white light, and trypsin (TRY) showed no temporal difference. The peak phase of Lactate dehydrogenase (LDH) under green, yellow, and red lights moved to the left compared to that under white light. In the five spectral environments, red and yellow light affected the peak of digestive enzyme activity earlier. The peak phase of pyruvate kinase (PK) shifted to the left under green, blue, and red lights. The secretion of hexokinase (HK) was observed under green, blue, and yellow lights, and its peak phase shifted to the left compared to that under white light. In five spectral environments, green and blue lights affected the peak of metabolic enzymes substantially. The melatonin level in the serum of Sebastes schlegelii was low during the day and high at night. Moreover, cortisol decreased during the day and began to diminish continuously after reaching its highest value at midnight. Changes in the peak phase indicate variations in circadian rhythms. The above results show that different spectra can influence the physiological metabolic rhythm of fish. Thus, future research should fully consider the biological effect of spectra on cultured organisms and then formulate appropriate lighting conditions. |
| Key words: Sebastes schlegelii spectra circadian rhythm digestion and metabolism stress |
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