| 摘要: |
| 光作为一个复杂的环境因子，对鱼类的孵化、摄食、生长及视觉均产生直接或间接的影响。为了探究不同LED光谱对许氏平鲉视网膜结构和视网膜各层细胞数目和视网膜各层厚度的影响，本文选取1000尾许氏平鲉幼鱼（体质量为38.80 ± 0.43 g、体长为10.20 ± 0.17 cm）分别养殖在五种光谱处理组中[蓝光（λ450 nm）、绿光（λ525 nm）、黄光（λ590 nm）、白光（λ460 nm）、红光（λ 630 nm）]，将白光组设为对照组并对其进行监测取样，结果表明：不同的LED光谱对许氏平鲉的视网膜结构没有明显的影响，但各个光谱下视网膜细胞数略有差别，在5种不同光谱条件下，在单位面积（100 μm*100 μm）面积内，蓝光组内网层细胞数量（24.33 ± 2.08）显著低于白光组（P<0.05）；红光组外核层细胞数量（33.33 ± 3.51）显著低于除白光组外的其他各组（P<0.05），由各个光谱下不同层视网膜细胞数量对比可得，蓝光组与红光组数量最少，其他各层差异不大，综上所述，通过本研究得出在蓝光和红光下养殖的许氏平鲉幼鱼视网膜发育受光谱影响较大，因此白光、黄光与绿光养殖许氏平鲉视网膜发育较好，这些研究结果为人工养殖光环境的制定提供一定的理论参考。 |
| 关键词: 许氏平鲉 LED光谱 视网膜结构 视网膜 |
| DOI： |
| 分类号:S966.9 |
| 基金项目:国家自然基金（32202961）、辽宁省科学技术计划项目（2021JH2/10200011）、教育部重点实验室开放课题（202202）、大连市领军人才（2019RD12）、国家贝类产业技术体系岗位科学家项目（CARS-49） |
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| Effect of LED Spectrum on Retina Morphology of Sebastes schlegelii |
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WU yanling1, SUN fei1, CAI haowei1, LI xin1, LIU ying2, LIU songtao3, MA he3
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1.College of Marine Technology and Environment, Dalian Ocean University;2.College of Biosystems Engineering and Food Science, ZheJiang University;3.Dalian Ocean University
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| Abstract: |
| As a complex environmental factor, light has a direct or indirect impact on the hatching, feeding, growth and vision of fish. In order to investigate the effects of different LED spectra on the retinal structure, the number of cells in each layer and the thickness of each layer of the retina of Sebastes schlegelii , 1000 scorpionfish (body weight: 38.80 ±0.43 g, body length: 10.20 ± 0.17 cm) were selected and cultured in five spectral treatment groups [Blue light（λ450 nm), green light（λ525 nm), yellow light（λ590 nm), white light（λ 460 nm), red light（λ 630 nm）], and monitored and sampled. The results showed that different LED spectra had no significant effect on the retinal structure of Sebastes schlegelii, but the number of retinal cells in Sebastes schlegelii was slightly different under each spectrum. Under five different spectral conditions, the number of internal layer cells in blue group (24.33 ± 2.08) was significantly lower than that in white group (P < 0.05) in unit area (100 μm*100 μm). The number of cells in the outer nuclear layer of red light group (33.33 ± 3.51) was significantly lower than that in other groups except white light group (P < 0.05). According to the comparison of the number of retinal cells in different layers of each spectrum, the number of cells in blue light group and red light group was the least, and there was no significant difference in other layers. To sum up, the spectrum has no effect on the retinal structure of Sebastes schlegelii . However, it has a significant effect on the number of retinal cells in each layer: under the condition of blue light and red light, the number of retinal cells in each layer is less, and the difference between the other groups is small. Therefore, yellow light, green light and white light have better retinal development on juvenile Sebastes schlegelii . |
| Key words: Sebastes schlegelii LED spectrum Structure of retina retina |