| 引用本文: | 宋元照,李佳蕙,董晓晓,杜文勇,姜海滨,李贤,李军.小球藻对不同盐度海水养殖尾水的净化效果研究[J].海洋科学,2022,46(11):126-138. |
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| 小球藻对不同盐度海水养殖尾水的净化效果研究 |
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宋元照1,2, 李佳蕙2, 董晓晓2, 杜文勇1,2, 姜海滨2, 李贤3, 李军4
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1.上海海洋大学水产与生命学院, 上海 201306;2.山东省海洋资源与环境研究院, 山东 烟台 264006;3.中国海洋大学水产学院, 山东 青岛 266071;4.中国科学院海洋研究所, 山东 青岛 266071
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| 摘要: |
| 微藻处理养殖尾水已成为热点研究方向,有关一定盐度范围内海水养殖尾水的微藻处理研究较少。本试验调配了两种盐度(16和26)的海水养殖尾水,以空白组作对照,设置小球藻(Chlorella salina)初始接种密度梯度(5×105、1×106、2×106和3×106个/mL),研究小球藻对海水养殖尾水中不同形态氮和磷的去除效果。结果表明,小球藻在海水养殖尾水中生长良好,可有效去除尾水中的氮磷营养盐,16盐度组中各初始藻密度组对NH4+、NO3-和总溶解态氮(total dissolved nitrogen,TDN)的去除率分别为85.03%~85.87%、60.87%~63.70%和54.53%~57.64%,组间无显著差异(P>0.05);26盐度组中除5×105组外,其余藻密度组对NH4+、NO3-和TDN的去除率分别为87.23%~88.16%、56.70%~57.79%和53.31%~54.62%,且组间无显著差异(P>0.05),表明小球藻初始接种密度对尾水中氮盐的去除无显著影响。除5×105个/mL组外,16与26盐度组中对TDN的去除率无显著差异,表明盐度变化对氮的去除无影响。随着初始藻密度的升高,16和26处理组对总溶解态磷(total dissolved phosphorus,TDP)的去除率均上升,分别为76.13%~99.53%和63.72%~96.83%,表明藻初始接种密度的升高可促进尾水中磷的去除,且盐度升高没有影响小球藻对磷的去除。本研究获得了不同初始接种密度小球藻对一定盐度范围的海水养殖尾水的吸收利用特点,可为海水养殖尾水的生态化处理提供一定的理论基础。 |
| 关键词: 小球藻(Chlorella salina) 盐度 接种密度 海水 水产养殖尾水 |
| DOI：10.11759/hykx20220525005 |
| 分类号:S949 |
| 基金项目:国家重点研发计划资助项目(2020YFD0900600);现代农业产业技术体系专项(CARS-47);山东省农业重大应用技术创新项目(SD2019YY011) |
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| Study on the purification effect of Chlorella salina on mariculture wastewater at different salinities |
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SONG Yuan-zhao1,2, LI Jia-hui2, DONG Xiao-xiao2, DU Wen-yong1,2, JIANG Hai-bin2, LI Xian3, LI Jun4
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1.College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China;2.Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;3.Fisheries College, Ocean University of China, Qingdao 266001, China;4.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
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| Abstract: |
| Aquaculture wastewater treatment by microalgae has become a research hotspot, but few studies on mariculture wastewater treatment by microalgae in a certain salinity range have been published. In this work, we prepared two salinities (16 and 26) of mariculture wastewater, with four initial inoculation density gradients (5×105, 1×106, 2×106, and 3×106 cells/mL) of Chlorella salina (ToCL007) and blank as control. We investigated the effects of C. salina on the removal of nitrogen and phosphorus in different ratios in mariculture wastewater. Both the satisfactory growth of C. salina and its effective removal of nitrogen and phosphorus nutrients in the mariculture wastewater were observed. At a salinity of 16, removal rates of 85.03%-85.87%, 60.87%-63.70%, and 54.53%-57.64% for NH4+, NO3-, and total dissolved nitrogen (TDN) were measured in the initial algae density groups, respectively [no significant difference between groups (P>0.05)]. At a salinity of 26, except for the 5×105 group, removal rates of 87.23%-88.16%, 56.70%-57.79%, and 53.31%-54.62% for NH4+, NO3-, and TDN, respectively, were calculated in the other algae density groups [no significant differences among groups (P>0.05)]. This shows that the initial inoculation density had no significant effect on nitrogen removal in wastewater. Except for the 5×105 cells∙mL-1 group, there was no significant difference in TDN removal rates between salinities of 16 and 26, indicating that a change in salinity had no effect on nitrogen removal. With increasing initial algal density, the total dissolved phosphorus (TDP) removal rate increased for the 16 and 26 treatment groups, from 76.13% to 99.53% and 63.72% to 96.83%, indicating that increasing initial algal density promoted phosphorus removal in wastewater and increasing salinity did not inhibit phosphate removal by C. salina. In addition, the characteristics of assimilation and utilization of mariculture wastewater by C. salina with different initial inoculation densities were obtained, providing a theoretical basis for the ecological treatment of mariculture wastewater. |
| Key words: Chlorella salina salinity inoculation density seawater aquaculture wastewater |
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