| 摘要: |
| 以大黄鱼(Larimichthys crocea)为研究对象,通过研究急性低盐度胁迫对大黄鱼血清生化组分的影响,评估盐度骤降引起鱼体的应激反应。急性低盐度(15、8)胁迫下,在72 h的实验周期中,大黄鱼血清葡萄糖(GLU)含量整体显著下降(P<0.05);血清总蛋白(TP)、白蛋白(ALB)及球蛋白(GLB)含量在15盐度胁迫下呈波动变化,与对照组相比无显著性差异(P>0.05),8盐度胁迫下,TP含量呈显著下降后逐渐升高趋势;血清甘油三酯(TG)和总胆固醇(CHOL)含量在15盐度胁迫下均在初始阶段显著变化后逐渐恢复至对照组水平,而8低盐胁迫后二者含量均在实验后期(48 h和72 h)大幅度显著升高;血清中的丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)含量在低盐胁迫下均显著高于对照组水平,且呈现出盐度相关性。研究结果表明,急性低盐胁迫会引起鱼体应激反应,实际生产中应避免养殖环境盐度的剧烈变化。 |
| 关键词: 大黄鱼 急性低盐胁迫 应激反应 血清生化组分 |
| DOI：10.11759/hykx20201028001 |
| 分类号:S965.322 |
| 基金项目:福建省科技厅自然科学基金项目(2018J05062);福建省中科院STS计划配套项目(2017T3016);福建省教育厅中青年教师教育科研项目(JAT170658);宁德市科技计划项目(20170046);宁德师范学院校级专项资助计划科研项目(2019ZX406) |
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| Effects of a sudden drop in salinity on serum biochemical components in Larimichthys crocea |
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HE Liang-yin1,2, LI Jin-shou1, SHI Xiao-li1,2, ZHOU Feng-fang1,2, HUANG Wei-qing1,2
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1.College of Life Science, Ningde Normal University, Ningde 352100, China;2.Engineering Research Center of Mingdong Aquatic Product Deep-Processing, Ningde 352100, China
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| Abstract: |
| Serum biochemical components were investigated to evaluate the stress response of Larimichthys crocea to acute low salinity shock. L. crocea was exposed to 15‰ and 8‰ salinity stress, at different time points (3, 6, 12, 24, 48, and 72 h) and compared with a control group. Serum glucose levels decreased significantly in the 15‰ and 8‰ groups of L. crocea within 72 h (P<0.05) compared to the 23‰ group. The total protein (TP), albumin, and globulin levels in the 15‰ group fluctuated during the 72 h and were not significantly different from the control, whereas the TP level dropped significantly in 3 h (P<0.05) but increased gradually under the 8‰ salinity stress. Triglyceride (TG) and cholesterol (CHOL) levels changed significantly during the initial stage, then gradually returned to the control level in the 15‰ group; however, the levels of TG and CHOL increased significantly during the later period under the 8‰ salinity stress. Alanine aminotransferase and aspartate aminotransferase levels were significantly higher than those in the control group under the low salinity stress, and a correlation with salinity was observed for both components. These results suggest that acute low salinity stress causes a stress response in L. crocea; thus, drastic changes in salinity should be avoided during farming practices. |
| Key words: Larimichthys crocea acute low salinity stress stress response serum biochemical components |
