引用本文:	赵珍,王宝杰,刘梅,蒋克勇,王雷.过硫酸氢钾复合物对对虾养殖底泥硝化作用、氨氧化微生物丰度和群落结构的影响[J].海洋科学,2021,45(11):54-61.

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过硫酸氢钾复合物对对虾养殖底泥硝化作用、氨氧化微生物丰度和群落结构的影响
赵珍^1,2, 王宝杰^1,3, 刘梅^1,3,4, 蒋克勇^1,3, 王雷^1,3,4
1.中国科学院海洋研究所中国科学院实验海洋生物学重点实验室, 山东青岛 266071;2.中国科学院大学, 北京 100049;3.中国科学院海洋大科学中心, 山东青岛 266071;4.青岛海洋科学与技术国家实验室海洋生物学与生物技术功能实验室, 山东青岛 266237

摘要:

采用在模拟池塘中投放过硫酸氢钾复合物（KMPS）进行对比实验的方法，探究KMPS对养殖底质硝化作用的影响。通过对氨氮和亚硝态氮含量的检测，探究对不同时期氮素转化的影响，低频率高剂量投放组中的氨氮和亚硝态氮含量显著降低，而高频率低剂量组中氨氮和亚硝态氮的含量显著上升。高频率低剂量KMPS的投放使氨氧化古菌（AOA）和氨氧化细菌（AOB）丰度降低，而低频率高剂量KMPS的投放不会造成丰度降低，而且还表现出部分时期AOB丰度的上升。进一步对群落结构进行分析发现KMPS的投放使AOA群落中Nitrosopumilu属相对丰度降低，Nitrososphaera属的相对丰度上升，这种相对丰度的变化与KMPS的投放方式无关；但AOB群落受到KMPS投放方式的影响，低频率高剂量的KMPS投放下AOB群落优势属相对丰度显著提高。以上结果均说明低频率高剂量KMPS的投放起到了促进底质硝化作用的效果。同时，可为KMPS用于对虾养殖池塘底质改良开辟一个新的途径。

关键词: 过硫酸氢钾复合物硝化作用 amoA基因丰度微生物群落结构

DOI：10.11759/hykx20210228002

分类号:S968.22

基金项目:国家重点研发计划课题（2019YFD0900401）；中国科学院科技支撑项目（2019T3035）

Effects of potassium monopersulfate on the nitrification, abundance, and community structure of ammonia-oxidizing microorganisms in shrimp culture sediments

ZHAO Zhen^1,2, WANG Bao-jie^1,3, LIU Mei^1,3,4, JIANG Ke-yong^1,3, WANG Lei^1,3,4

1.CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;4.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China

Abstract:

The effects of potassium monopersulfate (KMPS) on the nitrification of aquaculture substrates were investigated by adding KMPS into a simulated pond. To investigate the effects of KMPS on nitrogen conversion, ammonia nitrogen and nitrite nitrogen contents were measured. The contents of ammonia nitrogen and nitrite nitrogen in the low-frequency and high-dose groups were significantly decreased, whereas those in the high-frequency and low-dose groups were significantly increased. The application of high-frequency and low-dose KMPS reduced the abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB), whereas the application of low-frequency and high-dose KMPS increased the abundance of AOB in some periods. Further analysis of community structures showed that the relative abundance of Nitrosopumilu decreased, whereas that of Nitrososphaera increased in AOA due to the application of KMPS; this change was independent of the application mode of KMPS. However, the AOB community was affected by the application mode of KMPS, and the relative abundance of dominant genera in AOB significantly increased under low-frequency and high-dose KMPS. These results indicate that the application of low-frequency and high-dose KMPS can promote sediment nitrification. This study can also open a new possibility for KMPS to be used for aquaculture sediment improvement.

Key words: Potassium monopersultate nitrification amoA gene abundance microbial community structure