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引用本文:刘凯旋,岳欣,刘保忠.文蛤鳃组织内共生菌分离鉴定和多样性分析[J].海洋科学,2020,44(8):178-185.
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文蛤鳃组织内共生菌分离鉴定和多样性分析
刘凯旋1,2,3, 岳欣1,2,4, 刘保忠1,2,4,3
1.中国科学院海洋研究所, 山东 青岛 266071;2.中国科学院实验海洋生物学重点实验室, 山东 青岛 266071;3.中国科学院大学, 北京 100049;4.青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室, 山东 青岛 266237
摘要:
为探究文蛤(Meretrix petechialis)鳃组织内共生菌群的群落组成及结构,采用纯培养方法,对文蛤鳃组织的内共生菌进行了分离、纯化和16S rRNA鉴定,共筛选到215株细菌,分属于变形菌门、厚壁菌门、拟杆菌门和放线菌门4个门级类群中,其中变形菌门是实现纯培养菌株数量最多的类群。基于16S rRNA序列信息,进一步对变形菌门的系统演化关系进行了分析,结果显示不同纲级类群的菌株分别聚集在了相应的分支内。另外,对获得的具有潜在抑菌活性或致病性的共生细菌进行了探讨,为后续对文蛤鳃内生菌的深入研究奠定了基础。
关键词:  文蛤(Meretrix petechialis)  鳃组织  共生菌  多样性
DOI:10.11759/hykx20200319002
分类号:Q344
基金项目:中国科学院战略性先导科技专项(XDA24030304);浙江省科技计划项目(2018F10015)
Isolation, identification, and diversity analysis of endosymbiotic bacteria in gill tissue of Meretrix petechialis
LIU Kai-xuan1,2,3, YUE Xin1,2,4, LIU Bao-zhong1,2,4,3
1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;3.University of Chinese Academy of Sciences, Beijing 100049, China;4.Marine Biology and Biotechnology Laboratory, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
Abstract:
To explore the community composition and structure of endosymbiotic bacteria in the gill tissues of the clam Meretrix petechialis, a total of 215 strains of endosymbiotic bacteria were isolated and purified from the gills using pure culture method and then identified based on 16S rRNA sequencing. These strains were classified into four phyla: Proteobacteria, Firmicutes, Bacteroidetes, and Actinobacteria, among which Proteobacteria was the group with the largest number of the isolated strains. Based on the 16S rRNA sequencing information, the phylogenetic relationship of Proteobacteria was further analyzed. The phylogenetic tree showed that the strains of different class groups were clustered in the corresponding branches. In addition, we discussed the potential antibacterial activity or pathogenicity of the symbiotic bacteria. This study provides a reference for further studies of the endosymbiotic microbial community in M. petechialis.
Key words:  Meretrix petechialis  gill  endosymbiont  bacterial diversity
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