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引用本文:蔡丽希.一株芘降解菌Bacilluscereus Py5的分离鉴定[J].海洋科学,2018,42(11):29-34.
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一株芘降解菌Bacilluscereus Py5的分离鉴定
蔡丽希
莆田学院基础医学部, 福建 莆田 351110
摘要:
为了研究蜡状芽孢杆菌的筛选、鉴定及对四环多环芳烃芘的降解性能。通过富集驯化培养,从红树林污泥中分离到一株以芘作为唯一碳源与能源的高效菌株PY5,经过形态观察、生理生化与16S rDNA鉴定,PY5属于蜡状芽孢杆菌(Bacillus cereus),同时对该菌株降解芘性能进行了系统研究。结果表明,B.cereus对50 mg/L芘的21 d降解率为65.8%,投加Tween-80、葡萄糖、水杨酸、Fe3+、Mn2+等基质可以不同程度强化降解芘的能力,其中投加葡萄糖+Fe3++Mn2+的混合液对B.cereus降解芘的强化效果最好,21 d的芘降解率可达到81.4%,相对于不加共代谢底物时提升15.6%,表明外加C源与酶活诱导因子对高分子质量多环芳烃污染降解具有一定的促进作用。
关键词:    红树林  多环烷烃  降解  共代谢
DOI:10.11759/hykx20180920003
分类号:Q939.9;X172
基金项目:2013年莆田市科技计划项目[2013S01(4)]
Isolation and characterization of a pyrene-degrading bacterial strain Bacilluscereus Py5
CAI Li-xi
Department of Basic Medical Science, Putian University, Putian, 351100
Abstract:
The screening, identification of Bacillus cereu and its biodegradation characteristics of pyrene were investigated. A bacterial strain named PY5 which can utilize pyrene as sole carbon and energy source was isolatedfrom the sediment of mangrove area. The strain was identified as Bacillus cereus according to thephylogenetical analyses of 16S rDNA sequence. B.cereus PY5 showed high degradation capability with 65.8% of 50 mg/L pyrene within 21days. The adding of Tween-80、glucose、salicylic acid、Fe3+、Mn2+ can accelerate the degradation of pyrene by B. cereus. Degradation rate under the glucose + Fe3+ + Mn2+ mixture supplementation was 81.4% after 21 days, enhanced 15.6% compare to the condition of without cometabolism degradation substrate. The results also showed that the C source additionand the enzyme activity inducible factorsare feasible for the degradation of high molecular weight polycyclic aromatic hydrocarbons.
Key words:  pyrene  mangrove  polycyclic aromatic hydrocarbons(PAHs)  biodegradation  cometabolism
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