| 引用本文: | 王淑贤,韦章良,贾 睿,张建恒,霍元子,于克锋,何培民.绿潮藻浒苔微生物高效降解及生物乙醇制备[J].海洋科学,2017,41(1):76-82. |
| |
|
| 本文已被：浏览 1790次 下载 1560次 |
码上扫一扫！ |
|
|
| 绿潮藻浒苔微生物高效降解及生物乙醇制备 |
|
王淑贤1,2, 韦章良3, 贾 睿1,2, 张建恒4, 霍元子1,2, 于克锋1,2, 何培民1,2
|
|
1.上海海洋大学 水产与生命学院;2.上海海洋大学 水域环境生态上海高校工程研究中心;3.中国科学院南海海洋研究所 热带海洋生物资源与生态重点实验室;4.上海海洋大学 海洋科学学院
|
|
| 摘要: |
| 从绿潮藻暴发海域的底泥和腐烂浒苔(Ulva prolifera)中, 筛选出一株可高效降解浒苔纤维素的微生物菌株(F菌株), 通过刚果红染色实验以及真菌的ITS分析, 鉴定为曲霉属真菌。以浒苔为诱导培养基制备F菌株的粗酶液, 测得其滤纸纤维素酶活为34.79 U/mL。在发酵实验中, 当酶解条件为6%的粗酶液添加量、反应温度为38℃、反应时间为60 h、pH为6.8时, 浒苔纤维素降解效果最好, 酶解液发酵乙醇产量最高达到28.98 g/L。 |
| 关键词: 浒苔(Ulva prolifera) 纤维素降解菌 发酵 生物乙醇 |
| DOI：10.11759/hykx20160423003 |
| 分类号: |
| 基金项目:国家自然科学基金(41576163); 国家重点研发计划项目(2016YFC1402105); 国家海洋局公益性科研专项(201205010,201105023); 科技支撑计划项目(2012BAC07B023) |
|
| Screening and characterization of a newly isolated strain for microbiological degradation of Ulva prolifera and bioethanol production |
|
WANG Shu-xian,WEI Zhang-liang,JIA Rui,ZHANG Jian-heng,HUO Yuan-zi,YU Ke-feng,HE Pei-min
|
| Abstract: |
| Ulva prolifera been the dominant species in the huge annual biomass accumulation during green algae bloom in Yellow Sea since 2007. This species can be used for energy utilization by fermentation under certain conditions. In this study, we identified a highly efficient cellulose-decomposing microorganism by screening the rotten algae and employed it as a raw material to manufacture bioethanol. We studied its cellulose activity and the ability to decompose U. prolifera at different reaction temperatures, times. pH, and dosages of crude enzyme. We identified the strain with highest efficiency in cellulose-decomposition using Congo red staining method and ITS and termed it as the F strain (34.79U/mL). We also found that the optimum conditions for the fermentation of U. prolifera were as follows: crude enzyme, 6%; treating temperature, 38°C; treating time, 60 h; and pH, 6.8. Under these optimum conditions bioethanol yield of up to 28.98g/L was achieved. |
| Key words: Ulva prolifera cellulose-decomposing microorganisms fermentation bioethanol |