| 摘要: |
| 褐藻寡糖具有多种生理活性, 本研究通过酶解与分级条件的优化, 获得了低分子质量褐藻寡糖的酶法制备工艺, 并在体外模型上评价了不同分子质量组分的抗氧化活性。结果表明, 优化后的褐藻寡糖制备条件为: 底物浓度1.20%, 酶底比4.35 U/mg, 酶解时间4h。进一步采用乙醇沉淀和超滤分离后, 获得了重均分子质量分别为0.84、1.40、2.25和34.56 kDa的4种组分, 其得率分别为50.82%、5.15%、11.48%和12.00%。各组分均具有一定的还原能力, 可有效清除DPPH和羟自由基, 其中低分子质量组分A的抗氧化活性最为显著, 其清除羟自由基的能力与维生素C相近。 |
| 关键词: 褐藻寡糖 制备工艺 乙醇沉淀 超滤 抗氧化 |
| DOI：10.11759/hykx20161211 |
| 分类号: |
| 基金项目:国家海洋公益性行业科研专项(201305007); “十二五”国家科技支撑项目(2012BAD33B06) |
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| Efficient preparation of alginate oligosaccharides by enzymatic hydrolysis and evaluation of antioxidant activities in vitro |
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ZHANG Ming-jie,LI Heng,JIANG Min,HAO Yao,XU Zheng-hong,SHI Jin-song
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| Abstract: |
| Alginate oligosaccharides are characterized by various biological activities. In this study, we developed a technique for the efficient preparation of alginate oligosaccharides by optimizing the process conditions for enzymatic hydrolysis and separation. We evaluated the antioxidant activities of the obtained alginate hydrolysates in vitro. We found the optimized conditions for alginate oligosaccharides preparation to be as follows: a substrate concentration of 1.2%, a ratio of enzyme to substrate dosage of 4.35 U·mg-1, and a hydrolysis time of 4 h. Through successive processes of ethanol precipitation and ultrafiltration, we obtained four constituents with average molecular weights of 0.84, 1.40, 2.25, and 34.56 kDa, respectively. The yields of these four constituents were 50.82%, 5.15%, 11.48%, and 12.00%, respectively. Our in vitro antioxidation study results show that the constituent A (Mw 0.84 kDa) demonstrated the most significant antioxidant activity and the hydroxyl radical scavenging ability was similar to that of vitamin C. |
| Key words: alginate oligosaccharides preparation procedure ethanol precipitation ultrafiltration antioxidant activity |
