引用本文:	林思恒,吴富村,张国范.高温胁迫下皱纹盘鲍不同养殖群体心率变化比较[J].海洋科学,2016,40(11):84-90.

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高温胁迫下皱纹盘鲍不同养殖群体心率变化比较
林思恒^1,2,3,4, 吴富村^1,3,4, 张国范^1,3,4
1.中国科学院海洋研究所实验海洋生物学重点实验室;2.中国科学院大学;3.海洋科学与技术国家实验室海洋生物学与生物技术功能实验室;4.中国科学院海洋研究所海洋生态养殖技术国家地方联合工程实验室

摘要:

高温是海水贝类度夏死亡的环境诱因之一。本研究应用一种非损伤性的心率检测方法, 检测两个皱纹盘鲍养殖群体在高温胁迫条件下心率等生理指标的变化, 尝试以心率变化指标比较这两个群体高温耐受能力。由于高温胁迫下皱纹盘鲍的心率随温度变化的关系符合阿伦尼乌斯(Arrhenius)公式, 且心率随温度上升呈先上升后下降趋势, 该研究通过计算两者直线拟合拐点即阿伦尼乌斯拐点温度(ABT,Arrhenius break temperatures)指标, 用以指示皱纹盘鲍温度耐受程度。以此法对皱纹盘鲍两个群体(高温耐性, 对照)各17 个个体进行了测定分析, 结果表明: 两个群体间的ABT 存在显著差异, 高温耐性组的皱纹盘鲍的ABT 显著高于对照组(P<0.05); 个体ABT指标的高低与测定个体的壳高呈正相关(P<0.05)。本研究首次将探讨了高温胁迫下皱纹盘鲍心率变化规律, 并以ABT为指标分析比较了两个皱纹盘鲍养殖群体间高温耐受能力, 结果对研究皱纹盘鲍和其它贝类温度胁迫下生理响应及抗逆选育具一定借鉴意义。

关键词: 皱纹盘鲍(Haliotis discus hannai) 心率测定高温胁迫 ABT

DOI：10.11759/hykx20160328003

分类号:

基金项目:国家自然科学基金项目(31302184); 贝类产业技术体系项目(CARS-48); 泰山攀登计划项目

Variation in cardiac response to thermal stress in two different cultured populations of Pacific abalones

LIN Si-heng,WU Fu-cun,ZHANG Guo-fan

Abstract:

High water temperature is one of the most important reasons for the low summer survival rate of Pacific abalones (Haliotis discus hannai). The temperature that induces cardiac failure in abalone is known as the Arrhenius break temperature (ABT). Survival indicators of Pacific abalones can be obtained by measuring their heart rate. In this study, heart rates were measured using a non-invasive method. We then obtained ABTs as biomarkers and used them to determine the heat resistance of abalones with high accuracy and speed. Abalone heart rates rose as the temperature increased and then dropped abruptly. In this study, we measured the heart rate in 17 resistant abalones and 17 contrast abalones from two different groups. The results indicate that the ABT of the resistant abalones seemed higher (P<0.05) than that of the contrast abalones. In addition, there was a significant (P<0.05) positive correlation between abalone shell height and ABT. For the first time, we determined the heat resistance of Pacific abalones by measuring heart rate and verified the feasibility of the method.

Key words: Haliotis discus hannai measuring heart rate thermal stress Arrhenius break temperatures