| 摘要: |
| 在实验室控制条件下, 研究了无机磷磷酸二氢钠(NaH2PO4)及3种有机磷源三磷酸腺苷二钠盐(adenosine disodium triphosphate, ATP)、β-甘油磷酸二钠(sodium β-glycerophosphate, G-P)和D-葡萄糖-6-磷酸(D-Glucose 6-phosphate, D-G-6-P)对杜氏盐藻(Dunaliella salina)生长及PSII 系统的影响。结果表明,杜氏盐藻在ATP和NaH2PO4的磷环境中生长迅速, 最大比生长速率(μmax)分别为(0.736±0.0158)/d和(0.667±0.0553)/d; 而β-甘油磷酸二钠和D-葡萄糖-6-磷酸培养条件下盐藻生长则具有滞后效应, μmax分别为(0.232±0.0114)/d和(0.31±0.0077)/d。ATP和NaH2PO4作为磷源时, 盐藻最大电子传递效率(ETRmax)和最大饱和光强(Ik)显著高于β-甘油磷酸二钠和D-葡萄糖-6-磷酸处理组(P<0.05), 而NPQ则呈相反。JIP-test参数可知, 单位反应中心吸收的光能(ABS/RC)、t=0时单位反应中心捕获的用于还原QA的能量(TR0/RC)和最大光化学效率(Φ>P0)在各组间差异不显著(P>0.05), 但β-甘油磷酸二钠和D-葡萄糖-6-磷酸处理组单位反应中心耗散掉的能量(DI0/RC)显著增加(P<0.05), ψ0和ΦE0显著降低(P<0.05)。表明β-甘油磷酸二钠和D-葡萄糖-6-磷酸作为磷源时盐藻光合系统反应活性中心(RC)部分关闭, 反应活性中心的数量(RC/CS0)减少,PSⅡ受体侧电子传递受到影响, 能量耗散效率提高。综上可知, 杜氏盐藻均能利用无机磷和有机磷作为磷源供其生长, 但ATP作为磷源使得盐藻在最短时间进入对数期, 生物量显著提高(P<0.05)。 |
| 关键词: 杜氏盐藻(Dunaliella salina) 磷 叶绿素荧光 PSⅡ 生长 |
| DOI：10.11759//hykx20151204001 |
| 分类号: |
| 基金项目:甘肃省第九批科技计划项目(1009FTGG017); 甘肃省科技支撑计划—社会发展类项目(1604FKC090) |
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| Response of growth and PhotosystemⅡof Dunaliella salina on different phosphorus sources |
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YANG Song-qi,SHI Shao-hua,WANG Li-juan,XU Wei-min,LUO Guang-hon
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| Abstract: |
| The effects on the growth and PS Ⅱ of Dunaliella salina under inorganic and organic phosphorus sources, including NaH2PO4, ATP, sodium β-glycerophosphate (G-P), and D-Glucose 6-phosphate (D-G-6-P), were investigated. The results showed that D. salina could grow rapidly under ATP and NaH2PO4; the maximum growth rate (μmax) was 0.736±0.0158 and 0.667±0.0553 per day, respectively. However, a hysteresis phenomenon was discovered under G-P and D-G-6-P, and the μmax were 0.232±0.0114 and 0.31±0.0077 per day respectively, which was markedly lower than in the ATP and NaH2PO4 controls (P<0.05). The value of ETRmax and Ik under ATP and NaH2PO4 were significantly higher than those in the G-P and D-G-6-P controls (P<0.05), while the value of NPQ was the reverse. JIP test parameters showed that ABS/RC, TR0/RC, and ΦP0 did not differ significantly among groups (P>0.05); however, DI0/RC was increased, whereas ψ0 and ΦE0 were decreased significantly under G-P and D-G-6-P conditions (P<0.05). These results demonstrated that RC could close partially, RC/CS0 decreased, electron transport on the acceptor side of photosystemⅡwas blocked, and the efficiency of dissipation of energy increased when the phosphorus sources were G-P and D-G-6-P. In conclusion, D. salina could utilize both dissolved inorganic phosphate (DIP) and dissolved organic phosphorus (DOP), and ATP had a greater effect on growth than other organic phosphorus sources, which was reflected in the growth in the logarithmic phase at the shortest time and the significant increase in biomass in the presence of ATP (P<0.05). |
| Key words: Dunaliella salina phosphorus chlorophyll fluorescence photosystemⅡ (PSⅡ) Growth |
