| 摘要: |
| 以海水青鳉(Oryzias melastigma)胚胎为研究对象, 比较了120#燃料油分散液(water-accommodated fractions, WAFs)与乳化液(biologically enhanced water-accommodated fractions, BE-WAFs)的急性毒性效应, 并研究了不同浓度(40、100、250 mg/L)下WAFs、BE-WAFs对胚胎内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽硫转移酶(GST)活性的影响。结果表明: 在受到石油烃的氧化胁迫后, 海水青鳉胚胎内3 种抗氧化酶活性变化明显。随着石油烃浓度的升高和暴露时间的延长, 3种酶表现出程度不同的诱导效应和抑制效应。其中受石油烃污染影响最为明显的为SOD 酶; 而GST酶则对消油剂单独暴露表现较为敏感。实验证明, 海水青鳉体内SOD酶活性对石油烃污染反应最为敏感, 适合作为监测石油烃污染程度的生物标志物。 |
| 关键词: 海水青鳉(Oryzias melastigma) 石油烃 消油剂 抗氧化酶 120#燃料油 |
| DOI：10.11759/hykx20151224001 |
| 分类号: |
| 基金项目:国家自然科学基金项目(41276105/D0608); 交通运输部应用基础研究项目(2013329225250); 中央高校基本科研业务费(3132015081) |
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| Effects of dispersant and #120 fuel oil exposure on antioxidant enzyme activity of Oryzias melastigma embryos |
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GAO Xiang,DING Guang-hui,QIAN Yi-ting,JIANG Ling-ling,XIONG De-qi
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| Abstract: |
| In this paper, we consider the acute toxicities of #120 fuel oil water-accommodated fractions (WAFs) and biologically enhanced water-accommodated fractions (BE-WAFs) on Oryzias melastigma embryos. Specifically, in our study, we investigated the effects of WAFs and CE-WAFs with different concentrations on the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST). The results show that the activities of SOD, CAT, and GST vary significantly after experiencing oxidative stress from petroleum hydrocarbons. With increasing the TPH concentration and duration of exposure, the activities of SOD, CAT, and GST showed various degrees of inductive and inhibitory effects. Of the three enzymes, the activity of SOD varied most significantly when exposed to petroleum hydrocarbons. GST also showed sensitivity when exposed to a dispersant alone. These study results indicate that the SOD in O. melastigma has the highest sensitivity to pollution from petroleum hydrocarbons and is suitable for use as a biomarker for monitoring marine oil pollution. |
| Key words: Oryzias melastigma petroleum hydrocarbons dispersant antioxidant enzyme #120 fuel oil |
