引用本文:	仇登高,徐世宏,刘鹰,宋昌斌,迟良,王顺奎,于凯松.超声成像技术在大西洋鲑早期性别及发育期鉴别的应用研究[J].海洋科学,2016,40(6):23-29.

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超声成像技术在大西洋鲑早期性别及发育期鉴别的应用研究
仇登高^1,2,3, 徐世宏^1,3, 刘鹰^1,3,4, 宋昌斌⁵, 迟良¹, 王顺奎⁶, 于凯松⁶
1.中国科学院海洋研究所;2.福建省水产研究所福建省海洋生物增养殖与高值化利用重点实验室;3.海洋生态养殖技术国家地方联合工程实验室;4.大连海洋大学,;5.中国科学院半导体研究所;6.山东东方海洋科技股份有限公司

摘要:

采用KX-5100 便携式B 超机(7.5MHz 高频线阵探头)对273 尾1 龄或2 龄大西洋鲑(Salmo salar)的性别及性腺发育阶段进行了检测, 同时对检测的大西洋鲑进行了解剖和取样, 利用组织学观察法验证了超声成像技术对大西洋鲑雌雄性别和发育阶段的鉴别结果。研究结果显示: 利用超声成像技术鉴别雌、雄大西洋鲑的平均准确率为86%, 且鉴别雌鱼的准确率(93%)较雄鱼(81%)高。超声成像技术鉴别Ⅱ期+Ⅲ期、Ⅳ期、Ⅴ期卵巢的准确率分别为87%、92%和100%, 鉴别Ⅱ期、Ⅲ期、Ⅳ期、Ⅴ期精巢的准确率分别为64%、71%、86%和84%。总体看来, 超声成像技术鉴别早期性腺(Ⅱ期~Ⅲ期)发育的准确率较低, 仅为75%, 而对晚期性腺(Ⅳ期~Ⅴ期)发育鉴别的准确率较高, 达到89%。

关键词: 超声成像技术大西洋鲑(Salmo salar) 早期性别鉴别性腺发育阶段

DOI：10.11759//hykx20141003001

分类号:

基金项目:国家自然科学基金项目(31472312, 41306152, 31402283); 国家“十二五”科技支撑计划项目(2014BAD08B09); 江苏省重点研发项目(BE2015325)

The application of ultrasound imaging technology in the early determination of sex and gonadal development in Atlantic salmon (Salmo salar)

QIU Deng-gao,XU Shi-hong,LIU Ying,SONG Chang-bin,CHI Liang,WANG Shun-kui,YU Kai-song

Abstract:

Cultured Atlantic salmon (n = 273), aged 1–2 years were examined using an ultrasound device (KX-5100; with a 7.5-MHz linear probe) from April to December of 2012. Following ultrasound scanning, necropsy and sampling were performed and histological specimens were prepared to independently confirm ultrasound determinations of sex and developmental stages. Results showed that, on average, the accuracy of identification using ultrasound imaging technology was 86% in Atlantic salmon, and there was a higher accuracy of sex identification of females (93%) than males (81%). In addition, the accurate identification of stages Ⅲ, Ⅳ and Ⅴ in the ovaries of female fish were 87%, 92% and 100%, respectively, while the accurate identification of stages Ⅱ, Ⅲ, Ⅳ and Ⅴ in male testes were 64%, 71%, 86% and 84%, respectively. Overall, the accuracy of gonadal development in early stages (i.e., stages Ⅱ and Ⅲ) was lower (75%) than that of the later stages (i.e., stages Ⅳ and Ⅴ), which was 89%.

Key words: ultrasound imaging technology Salmo salar early sex determination gonadal development