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壳聚糖碱性氨基酸衍生物的合成及抑菌活性研究
黄勇1,2, 刘松1, 秦玉坤1, 邢荣娥1, 李克成1, 于华华1, 岳洋1, 李俨1, 李鹏程1
1.中国科学院海洋研究所;2.中国科学院大学
摘要:
利用接枝反应,采用碱性氨基酸修饰壳聚糖,制备壳聚糖赖氨酸衍生物、壳聚糖精氨酸衍生物、壳聚糖组氨酸衍生物,通过红外(FT-IR)、核磁(1H-NMR)、元素分析(EA)对其进行表征,同时研究了不同壳聚糖氨基酸衍生物的抑菌活性。结果表明,壳聚糖赖氨酸衍生物、壳聚糖精氨酸衍生物、壳聚糖组氨酸衍生物、壳聚糖对金黄色葡萄球菌的最低抑菌浓度(MIC)分别为320、160、320、640 μg/mL,对大肠杆菌的最低抑菌浓度(MIC)分别为320、320、320、640 μg/mL,三种壳聚糖氨基酸衍生物的抑菌活性均明显高于未修饰壳聚糖。通过引入碱性氨基酸增加壳聚糖的正电荷有利于提高其抑菌活性。
关键词:  壳聚糖  氨基酸;衍生物制备  抑菌活性
DOI:10.11759/hykx20160408001
分类号:Q
基金项目:中国科学院STS计划项目(KFJ-SW-STS-143);国家海洋公益性项目(201305016-2、201405038-2);NSFC-山东联合资助项目(U1406402-5)
Synthesis and antibacterial activities of basic amino acid chitosan derivatives
Yong Huang1,2, Song Liu1, Yukun Qin1, Ronge Xing1, Kecheng Li1, Huahua Yu1, Yang Yue1, Yan Li1, Pengcheng Li1
1.Institute of Oceanology,Chinese Academy of Sciences;2.University of Chinese Academy of Sciences
Abstract:
In this study,three basic amino acids were used to modify chitosan to improve the antibacterial activity of chitosan. Lysine-modified chitosan,arginine-modified chitosan and histidine-modified chitosan were synthesized, and their structures were characterized by Fourier-transform infrared (FT-IR), magnetic resonance spectra(1H-NMR), and elemental analysis(EA). Amino acids were grafted to chitosan successfully as the results showed. The antibacterial activities against Escherichia coli and Staphylococcus aureus were investigated. The MIC of Lysine-modified chitosan,arginine-modified chitosan and histidine-modified chitosan on Staphylococcus aureus was 320 μg/mL,160 μg/mL,320 μg/mL,640 μg/mL, respectively,and the MIC on Escherichia coli was 320 μg/mL,320 μg/mL,320 μg/mL,640 μg/mL respectively. Three kinds of basic amino acid chitosan derivatives presented better antibacterial activity than chitosan.
Key words:  Chitosan  amino acid  Synthesis of derivatives  antibacterial activity
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