| 摘要: |
| 利用接枝反应，采用碱性氨基酸修饰壳聚糖，制备壳聚糖赖氨酸衍生物、壳聚糖精氨酸衍生物、壳聚糖组氨酸衍生物，通过红外(FT-IR)、核磁(1H-NMR)、元素分析（EA）对其进行表征，同时研究了不同壳聚糖氨基酸衍生物的抑菌活性。结果表明，壳聚糖赖氨酸衍生物、壳聚糖精氨酸衍生物、壳聚糖组氨酸衍生物、壳聚糖对金黄色葡萄球菌的最低抑菌浓度（MIC）分别为320、160、320、640 μg/mL，对大肠杆菌的最低抑菌浓度（MIC）分别为320、320、320、640 μg/mL，三种壳聚糖氨基酸衍生物的抑菌活性均明显高于未修饰壳聚糖。通过引入碱性氨基酸增加壳聚糖的正电荷有利于提高其抑菌活性。 |
| 关键词: 壳聚糖 氨基酸；衍生物制备 抑菌活性 |
| DOI：10.11759/hykx20160408001 |
| 分类号:Q |
| 基金项目:中国科学院STS计划项目（KFJ-SW-STS-143）；国家海洋公益性项目（201305016-2、201405038-2）；NSFC-山东联合资助项目（U1406402-5） |
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| Synthesis and antibacterial activities of basic amino acid chitosan derivatives |
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Yong Huang1,2, Song Liu1, Yukun Qin1, Ronge Xing1, Kecheng Li1, Huahua Yu1, Yang Yue1, Yan Li1, Pengcheng Li1
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1.Institute of Oceanology,Chinese Academy of Sciences;2.University of Chinese Academy of Sciences
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| Abstract: |
| In this study，three basic amino acids were used to modify chitosan to improve the antibacterial activity of chitosan. Lysine-modified chitosan，arginine-modified chitosan and histidine-modified chitosan were synthesized, and their structures were characterized by Fourier-transform infrared (FT-IR), magnetic resonance spectra（1H-NMR）, and elemental analysis（EA）. Amino acids were grafted to chitosan successfully as the results showed. The antibacterial activities against Escherichia coli and Staphylococcus aureus were investigated. The MIC of Lysine-modified chitosan，arginine-modified chitosan and histidine-modified chitosan on Staphylococcus aureus was 320 μg/mL,160 μg/mL,320 μg/mL,640 μg/mL, respectively，and the MIC on Escherichia coli was 320 μg/mL,320 μg/mL,320 μg/mL,640 μg/mL respectively. Three kinds of basic amino acid chitosan derivatives presented better antibacterial activity than chitosan. |
| Key words: Chitosan amino acid Synthesis of derivatives antibacterial activity |