| 引用本文: | 崔翠菊,李 言,刘延岭,李晓捷,罗世菊,武瑞娜,王利芹,张壮志,孙 娟,田 鑫.运用分子标记辅助构建海带核心种质资源[J].海洋科学,2016,40(1):18-24. |
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| 运用分子标记辅助构建海带核心种质资源 |
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崔翠菊1, 李 言1, 刘延岭1, 李晓捷1, 罗世菊1, 武瑞娜1, 王利芹1, 张壮志1, 孙 娟1, 田 鑫2
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1.山东东方海洋科技股份有限公司, 国家海藻与海参工程技术研究中心, 山东省海藻遗传育种与栽培技术重点实验室;2.江西财经大学
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| 摘要: |
| 海带(Saccharina japonica)是一种具有重要经济和生态价值的大型海藻, 配子体是其种质资源保存的形式, 也是海带杂交育种、育苗的材料。为有效管理和评价海带种质资源, 初步构建了海带核心种质资源, 利用RAPD标记技术对海带“早厚成一号”的28份配子体进行了遗传学评价。采用UPGMA聚类法, 根据遗传相似性系数进行分组, 采用简单比例法进行取样(25%、35%、50%、65%)分析, 计算不同取样比例时的遗传多样性参数, 结合样本的生长状态和雌、雄比例, 综合评价遗传多样性的各种参数, 取样比例为50%时构建的核心种质库相对合理。本研究为海带品种(系)的核心种质的筛选和遗传学评价打下基础。 |
| 关键词: 海带(Saccharina japonica) RAPD 分子标记 核心种质 遗传多样性 |
| DOI：10.11759/hykx20150319001 |
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| 基金项目:国家科技支撑计划项目(2012BAD55G01); 国家高技术研究发展计划(863 计划)项目(2012AA10A406); 烟台市科技发展计划项目(2013LGS002) |
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| Establishment of core collection for Saccharina japonica using molecular markers |
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CUI Cui-ju1, LI Yan1, LIU Yan-ling1, LI Xiao-jie1, LUO Shi-ju1, WU Rui-na1, WANG Li-qin1, ZHANG Zhuang-zhi1, SUN Juan1, TIAN Xin2
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1.Shandong Oriental Ocean Sci-tech Co. Ltd, National Algae and Sea cucumber Project Technology Research Centre, Algae Genetic Breeding and Cultivation Technology Key Laboratory of Shandong Province;2.Jiangxi University of Finance and Economics
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| Abstract: |
| Kelp is a large alga with an important economic and ecological value. The gametophytes are the preserved materials for kelp germplasm resources, which are also used for kelp crossbreeding and seedling. For an effective management and evaluation of kelp germplasm resources, a core collection of germplasm was screened. Genetic evaluation of 28 gametophytes of “Zaohoucheng” variety was conducted by RAPD molecular marker technology, using the UPGMA clustering method, based on the genetic similarity coefficient of grouping, simple percentage sampling (25%, 35%, 50%, and 35%), and analysis and calculation of genetic diversity parameters of different sampling rates, combined with the growth status and proportion of males and females. The core collection at the sampling rate of 50% was relatively reasonable. These results laid the foundation for screening and genetic evaluation of core germplasm of kelp varieties (or lines). |
| Key words: Saccharina japonica RAPD molecular markers core germplasm genetic diversity |