| 摘要: |
| 为了评价琼枝麒麟菜多糖(EGP)包括粗提物(CEGP)及大于500 kDa 多糖组分(LEGP)抗呼吸道相关病毒活性。采用Reed-muench 法计算流感病毒H1N1、H3N2 和H5N3, 柯萨奇病毒CVB3 及呼吸道合胞病毒RSV-long 株滴度; 采用观察细胞病变效应(CPE)法, 确定CEGP 和LEGP 对犬肾细胞(MDCK),人宫颈癌细胞(HeLa)和人喉癌上皮细胞(HEp-2)的最大无毒浓度; 通过细胞病变观察法(CPE)及四甲基偶氮唑盐微量酶反应比色法(MTT 法)测定CEGP 和LEGP 抑制各病毒产生细胞病变的作用。结果表明:CEGP 和LEGP 对5 种常见的呼吸道病毒H1N1、H3N2 和H5N3, CVB3 和RSV 都有抑制作用, CVB3和RSV 对CEGP 和LEGP 更敏感; CEGP 和LEGP 有抗多种呼吸道病毒作用, 为抗呼吸道病毒新药的研发提供理论依据。 |
| 关键词: 琼枝麒麟菜多糖 抗病毒 呼吸道病毒 |
| DOI：10.11759/hykx20141128001 |
| 分类号: |
| 基金项目:广东省海洋渔业科技推广专项科技攻关与研发(A201301C06)资助 |
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| Anti-respirovirus activity of a Eucheuma gelatinae polysaccharide |
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| Abstract: |
| Objective: To evaluate the anti-respirovirus effects of crude Eucheuma gelatinae polysaccharides (CEGP) and large molecular weight components (MW > 500 kDa, LEGP). Method: The Reed-Muench method was used to calculate the titer of the influenza virus (H1N1, H5N3, and H3N2), coxsackie virus (CVB3), and the respiratory syncytial virus (RSV-long). The maximum no toxicity concentrations of CEGP and LEGP to each cell line (MDCK, HeLa, and HEp-2) were selected by the cytopathic effect (CPE) method. The antiviral effects of CEGP and LEGP were evaluated by the MTT and CPE methods. Results: Both CEGP and LEGP showed significant and similar inhibition effects on the influenza virus (H1N1, H5N3, and H3N2), CVB3, and RSV-long. CVB3 and RSV were more sensitive to EGP (CEGP and LEGP) compared with the influenza virus. Conclusion: CEGP and LEGP showed great potential in respirovirus inhibition. |
| Key words: Eucheuma gelatinae polysaccharides antiviral activity respirovirus |
