| 摘要: |
| 在春季, 以中肋骨条藻和海洋原甲藻培养中华哲水蚤, 以饥饿培养作为对照, 测定了饵料的脂肪酸和挥发性不饱和醛类的组成和含量、雌体的产卵率和卵的孵化率, 试图探讨藻类生化组成对桡足类繁殖的影响。结果表明, 中肋骨条藻单不饱和脂肪酸、?Σ16 脂肪酸的含量相对较高, 而海洋原甲藻则具有更高含量的总脂肪酸、多不饱和脂肪酸、Σω3、Σω6、Σ18 脂肪酸。中肋骨条藻和海洋原甲藻分别测得了6 种和3 种不饱和醛类, 二者醛类总含量分别为1438.95 μg/g 和114.58 μg/g。在饥饿的情况下,中华哲水蚤的产卵会停止。中华哲水蚤在中肋骨条藻和海洋原甲藻培养下并没有表现出产卵率和孵化率的差异, 一方面说明并非所有的硅藻都会对桡足类的繁殖产生抑制作用, 另一方面说明藻类进入桡足类体内以后并未因其生化组成的差异对卵的孵化产生影响。中肋骨条藻培养下中华哲水蚤具有更高的死亡率, 表明硅藻在浓度较高时会对桡足类的存活产生影响。 |
| 关键词: 中华哲水蚤 产卵率 孵化率 脂肪酸 不饱和醛 |
| DOI：10.11759/hykx20150212001 |
| 分类号: |
| 基金项目:国家重点基础研究发展计划(973)项目(2015CB452903,2011CB403604); 国家自然科学基金资助项目 (41476139, 41406159) |
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| Influence of two algal diets on the reproduction of the copepod Calanus sinicus based on a biochemical analysis of diets |
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| Abstract: |
| In a laboratory experiment with a duration of 24 days, the influence of two algal diets on egg production and hatching success of the copepod Calanus sinicus was studied. During the experiment, Skeletonema costatum and Prorocentrum micans were offered as diets at the same concentration and with starvation as the control. The diets were analyzed for their fatty acid and unsaturated aldehydes compositions. Comparatively, P. micans was rich in Σ18, Σω3, Σω6, polyunsaturated fatty acid, and total fatty acid, and S. costatum was rich in Σ16 and monounsaturated fatty acid. The total unsaturated aldehydes of S. costatum and P. micans were 1438.95 μg/g and 114.58 μg/g, respectively. C. sinicus terminated egg production after being placed in filtered sea water for a period of time. The egg production rate and hatching success of C. sinicus did not show significant differences based on a diet of S. costatum or P. micans, indicating that not all diatom diets have a negative effect on copepod reproduction. At the end of the experiment, C. sinicus survival rates were 61%, 81%, and 87% with diets of S. costatum, P. micans, or starvation, respectively. We considered that the nutritional imbalances of fatty acid composition and the production of unsaturated aldehydes in S. costatum might reduce the survival of C. sinicus. |
| Key words: Calanus sinicus egg production rate hatching success fatty acid unsaturated aldehydes |
